Western blot abreview for Anti-Fatty Acid Synthase antibody

Excellent
Abreviews
Application
Western blot
Sample
Mouse Tissue lysate - whole (Liver)
Loading amount
40 µg
Specification
Liver
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C

Other product details

Dilution
1/5000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C

Secondary antibody

Name
Non-Abcam antibody was used: Donkey Anti-Rabbit IgG ECL Antibody, HRP Conjugate
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000

Detection

Detection method
ECL+
Exposure
10 second(s)
Bands
Specific: 275 kDa
Positive control
Wild-type mouse liver lysate

Abcam user community

Verified customer

Submitted Mar 02 2011

Sign up