Western blot abreview for Anti-Fatty Acid Synthase antibody

Excellent
Abreviews
Application
Western blot
Sample
Mouse Tissue lysate - whole (muscle)
Loading amount
20 µg
Specification
muscle
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Dilution
1/3000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% BSA in TBST

Secondary antibody

Name
Non-Abcam antibody was used: HRP-conjugated goat anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000

Detection

Detection method
ECL+
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: ~260 kDa

Additional data

Additional Notes
Lane 1: gastrocnemius muscle, Lane 2: tibialis anterior muscle

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Submitted Mar 25 2011

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