Western blot abreview for Anti-Fatty Acid Synthase antibody

Excellent
Abreviews
Application
Western blot
Sample
Rat Tissue lysate - whole (Rat liver tissue)
Loading amount
140 µg
Specification
Rat liver tissue
Gel Running Conditions
Reduced Denaturing (6% gel. Sample was heated in laemli buffer at 70 C for 10 min before loading)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Dilution
1/1000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% BSA in TBS-T(0.1%)

Secondary antibody

Name
Non-Abcam antibody was used: Anti-rabbit IgG, HRP-linked Antibody
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/1000

Detection

Detection method
Pierce ECL West Pico
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 273 kDa

Additional data

Additional Notes
lane1: control rat
lane 2: control rats given plant extract

Mrs. Smitha Koshy

Verified customer

Submitted Aug 13 2012

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