Western blot abreview for Anti-Fatty Acid Synthase antibody

Good
Abreviews
Application
Western blot
Sample
Mouse Tissue lysate - whole (Liver)
Loading amount
35 µg
Specification
Liver
Gel Running Conditions
Reduced Denaturing (8% SDS-PAGE)
Blocking step
odyssey blocking buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 37°C

Other product details

Dilution
1/1000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: odyssey blocking buffer

Secondary antibody

Name
Non-Abcam antibody was used: Goat anti-Rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/4000

Detection

Detection method
ECL+
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 275 kDa Non-specific: 50 kDa
Positive control
Liver whole tissue lysate

Mr. Ahmed Nayef

Verified customer

Submitted Dec 26 2012

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