Western blot abreview for Anti-Fatty Acid Synthase antibody

Excellent
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (293T, HepG2, MEF)
Loading amount
20 µg
Specification
293T, HepG2, MEF
Gel Running Conditions
Reduced Denaturing (8%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 7% · Temperature: 20°C

Other product details

Dilution
1/2000
Incubation time
17 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBST

Secondary antibody

Name
Non-Abcam antibody was used: HRP-conjugated anti-rabbit secondary antibody
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/2000

Detection

Detection method
ECL
Exposure
1 minute(s) and 30 second(s)
Bands
Specific: 273 kDa Non-specific: 52 kDa
Positive control
HepG2

Additional data

Additional Notes
Lane 1 : 293T (Human embryonic kidney cells) whole cell extract.
Lane 2 : HepG2 (Human hepatocellular liver carcinoma cells) whole cell extract.
Lane 3 : MEF (Mouse embryonic fibroblast) whole cell extract.

Mr. Dongil Kim

Verified customer

Submitted Jan 15 2013

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