Western blot abreview for Anti-Fatty Acid Synthase antibody

Excellent
Abreviews
Application
Western blot
Sample
Mouse Tissue lysate - whole (Liver)
Gel Running Conditions
Reduced Denaturing (8%)
Loading amount
50 µg
Treatment
Western diet 12 weeks
Specification
Liver
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBST + 2.5% milk
Dilution
1/3000

Secondary antibody

Dilution
1/2000
Name
Non-Abcam antibody was used: Goat anti rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL
Exposure
1 second(s)
Bands
Specific: 273 kDa Non-specific: None kDa

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Submitted Sep 10 2018

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