Application
Western blot
Sample
Rat Tissue lysate - whole (rat liver)
Gel Running Conditions
Reduced Denaturing (7% SDS-PAGE)
Loading amount
20 µg
Specification
rat liver
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBST
Dilution
1/1000
Secondary antibody
Dilution
1/1000
Name
Non-Abcam antibody was used: anti-Rabbit IgG (H+L)--HRP
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL Pico
Exposure
3 minute(s) and 0 second(s)
Bands
Specific: 270 kDa Non-specific: 120, 90, 45 kDa
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Abcam user community
Verified customer
Submitted Sep 05 2016