Validated using a knockout cell line
Recombinant
RabMAb

Recombinant Anti-Fatty Acid Synthase antibody [EPR7465] - BSA and Azide free (ab227083)

Overview

  • Product name

    Anti-Fatty Acid Synthase antibody [EPR7465] - BSA and Azide free
    See all Fatty Acid Synthase primary antibodies
  • Description

    Rabbit monoclonal [EPR7465] to Fatty Acid Synthase - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cyt, ICC/IF, IHC-Frmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Fatty Acid Synthase aa 1-100. The exact sequence is proprietary.
    Database link: P49327

  • Positive control

    • HeLa, 293T, A549, SHSY-5Y and MOLT4 cell lysates; A549 cells
  • General notes

    Ab227083 is the carrier-free version of ab128856. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab227083 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

Applications

Our Abpromise guarantee covers the use of ab227083 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 273 kDa.
Flow Cyt Use at an assay dependent concentration.

ab199376 - Rabbit monoclonal IgG (Low endotoxin, Azide free), is suitable for use as an isotype control with this antibody.

 

ICC/IF Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Function

      Fatty acid synthetase catalyzes the formation of long-chain fatty acids from acetyl-CoA, malonyl-CoA and NADPH. This multifunctional protein has 7 catalytic activities and an acyl carrier protein.
    • Tissue specificity

      Ubiquitous. Prominent expression in brain, lung, and liver.
    • Sequence similarities

      Contains 1 acyl carrier domain.
    • Cellular localization

      Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
    • Information by UniProt
    • Database links

    • Alternative names

      • [Acyl-carrier-protein] S acetyltransferase antibody
      • [Acyl-carrier-protein] S malonyltransferase antibody
      • 3-hydroxypalmitoyl-[acyl-carrier-protein] dehydratase antibody
      • 3-oxoacyl-[acyl-carrier-protein] reductase antibody
      • 3-oxoacyl-[acyl-carrier-protein] synthase antibody
      • Enoyl-[acyl-carrier-protein] reductase antibody
      • FAS antibody
      • FAS_HUMAN antibody
      • FASN antibody
      • Fatty acid synthase antibody
      • MGC14367 antibody
      • MGC15706 antibody
      • OA 519 antibody
      • Oleoyl-[acyl-carrier-protein] hydrolase antibody
      • SDR27X1 antibody
      • Short chain dehydrogenase/reductase family 27X member 1 antibody
      see all

    Images

    • This WB data was generated using the same anti-Fatty Acid Synthase antibody clone, EPR7465, in a different buffer formulation (cat# ab128856).

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: Fatty Acid Synthase knockout HAP1 cell lysate (20 µg) 
      Lane 3: A549 cell lysate (20 µg)
      Lane 4: Hu liver tissue lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab128856 observed at 250 kDa. Red - loading control, ab18058, observed at 124 kDa.

      ab128856 was shown to specifically react with Fatty Acid Synthase when Fatty Acid Synthase knockout samples were used. Wild-type and Fatty Acid Synthase knockout samples were subjected to SDS-PAGE. ab128856 and ab18058 (loading control to Vinculin) were diluted at 1/1000 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

    • Overlay histogram showing A549 cells stained with ab128856 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab128856, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in A549 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab128856).

    • ab128856 staining Fatty Acid Synthase in human peritoneal tumor tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde and blocked with 1% BSA for 1 hour at room temperature. Samples were incubated with primary antibody (1/500) for 2 hours. An Alexa Fluor® 647-conjugated anti-rabbit IgG monoclonal (1/1000) was used as the secondary antibody.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab128856).

    • ab128856 at a 1/250 dilution staining Fatty Acid Synthase in A549 cells by immunofluorescence.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab128856).

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab128856).

    References

    This product has been referenced in:

    See 1 Publication for this product

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