Validated using a knockout cell line
Recombinant
RabMAb

Recombinant Anti-Fatty Acid Synthase antibody [EPR7466] (ab128870)

Submit a review Q&A (3)References (10)

Overview

  • Product name

    Anti-Fatty Acid Synthase antibody [EPR7466]
    See all Fatty Acid Synthase primary antibodies

  • Description

    Rabbit monoclonal [EPR7466] to Fatty Acid Synthase

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IP, IHC-P, Flow Cyt, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human Fatty Acid Synthase aa 2450-2550 (C terminal). The exact sequence is proprietary.

  • Positive control

    • HeLa, 293T and A549 cell lysates; Human liver tissue; A549 cells

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Applications

Our Abpromise guarantee covers the use of ab128870 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 273 kDa.
IP Use at an assay dependent concentration.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
ICC/IF 1/250 - 1/500.

Target

  • Function

    Fatty acid synthetase catalyzes the formation of long-chain fatty acids from acetyl-CoA, malonyl-CoA and NADPH. This multifunctional protein has 7 catalytic activities and an acyl carrier protein.

  • Tissue specificity

    Ubiquitous. Prominent expression in brain, lung, and liver.

  • Sequence similarities

    Contains 1 acyl carrier domain.

  • Cellular localization

    Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • [Acyl-carrier-protein] S acetyltransferase antibody
    • [Acyl-carrier-protein] S malonyltransferase antibody
    • 3-hydroxypalmitoyl-[acyl-carrier-protein] dehydratase antibody
    • 3-oxoacyl-[acyl-carrier-protein] reductase antibody
    • 3-oxoacyl-[acyl-carrier-protein] synthase antibody
    • Enoyl-[acyl-carrier-protein] reductase antibody
    • FAS antibody
    • FAS_HUMAN antibody
    • FASN antibody
    • Fatty acid synthase antibody
    • MGC14367 antibody
    • MGC15706 antibody
    • OA 519 antibody
    • Oleoyl-[acyl-carrier-protein] hydrolase antibody
    • SDR27X1 antibody
    • Short chain dehydrogenase/reductase family 27X member 1 antibody
    see all

Images

  • Western blot - Anti-Fatty Acid Synthase antibody [EPR7466] (ab128870)
    Western blot - Anti-Fatty Acid Synthase antibody [EPR7466] (ab128870)

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: Fatty Acid Synthase knockout HAP1 cell lysate (20 µg)
    Lane 3: A549 cell lysate (20 µg)
    Lane 4: Hu liver tissue lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab128870 observed at 250 kDa. Red - loading control, ab18058, observed at 124 kDa.

    ab128870 was shown to react with Fatty Acid Synthase in wild-type HAP1 cells along with additional cross reactive bands. No band was observed when Fatty Acid Synthase knockout samples were examined. Wild-type and Fatty Acid Synthase knockout samples were subjected to SDS-PAGE. ab128870 and ab18058 (loading control to Vinculin) were both diluted at 1/10,000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

  • Flow Cytometry - Anti-Fatty Acid Synthase antibody [EPR7466] (ab128870)
    Flow Cytometry - Anti-Fatty Acid Synthase antibody [EPR7466] (ab128870)
    Overlay histogram showing A549 cells stained with ab128870 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab128870, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in A549 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
  • Western blot - Anti-Fatty Acid Synthase antibody [EPR7466] (ab128870)
    Western blot - Anti-Fatty Acid Synthase antibody [EPR7466] (ab128870)
    All lanes : Anti-Fatty Acid Synthase antibody [EPR7466] (ab128870) at 1/10000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : 293T cell lysate
    Lane 3 : A549 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat-anti-rabbit HRP at 1/2000 dilution

    Developed using the ECL technique.

    Predicted band size: 273 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fatty Acid Synthase antibody [EPR7466] (ab128870)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fatty Acid Synthase antibody [EPR7466] (ab128870)

    ab128870, at a 1/100 dilution, staining Fatty Acid Synthase in paraffin embedded Human liver tissue by Immunohistochemistry.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-Fatty Acid Synthase antibody [EPR7466] (ab128870)
    Immunocytochemistry/ Immunofluorescence - Anti-Fatty Acid Synthase antibody [EPR7466] (ab128870)
    ab128870, at a 1/250 dilution, staining Fatty Acid Synthase in A549 cells by Immunofluorescence.
  • Other - Anti-Fatty Acid Synthase antibody [EPR7466] (ab128870)
    Other - Anti-Fatty Acid Synthase antibody [EPR7466] (ab128870)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

References

This product has been referenced in:

  • Che L  et al. Valine increases milk fat synthesis in mammary gland of gilts through stimulating AKT/MTOR/SREBP1 pathway‡. Biol Reprod N/A:N/A (2019). Read more (PubMed: 30985894) »
  • Su L  et al. Cideb controls sterol-regulated ER export of SREBP/SCAP by promoting cargo loading at ER exit sites. EMBO J 38:N/A (2019). Read more (PubMed: 30858281) »
See all 10 Publications for this product

Publishing research using ab128870? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1-3 of 3 Abreviews or Q&A

Question

I am interested in testing this product
thank you

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Answer

DISCOUNT CODE: xxxxxxxxxx
Expiration date: xxxxxxxxx
Value: $xxxxx

I am very pleased to hear you would like to accept our offer and test ab128870 in Pig. This code will give you $xxxxxxx off your next order before the expiration date. To redeem this offer, please submit an Abreview for Pig and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.


Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/abtrial.

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Question

Do you have a FASN (fatty acid synthase) antibody that will recognize pig (sus scrofa). I attached the pig amino acid sequence for FASN. thank you

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Answer

Thank you for contacting us.

I have looked through our catalogue and I have not found an anti-fatty acid synthase which is guarenteed to work in pig. However, I have found an antibody ab128870 (https://www.abcam.com/ab128870) who's immunogen shows 86.7% homology with that of pig and therefore it is likely that this antibody will work in pig.

Please do note that this antibody has not been tested in pig and therefore is not guarenteed to work. However, if you wish to test this antibody in pig I can offer you a testing discount.
Simply follow these easy steps below to apply for our AbTrial Program:

1. Reply to this email, letting us know you are interested in testing this product.

2. Our scientists will email you an inactive personal discount code for the value of the product.

3. Purchase and test the product at the regular price.

4. Submit your results, including your discount code in the additional notes section of your Abreview.

5. Once the Abreview is submitted, the discount code will become active.

6. Apply your discount code on your next order to receive that value off.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: www.abcam.com/abtrial.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Question

Nos han llegado los anticuerpos ab128870 y ab18180 y tenemos una duda acerca de su conservación. ¿Es necesario preparar el buffer o los anticuerpos están ya disueltos en él?
Muchas gracias.

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Answer

Gracias por contactarnos.



Los anticuerpos están ya disueltos en el buffer apropiado, lo que sí es importante seguir son las instrucciones para conservarlo en perfecto estado, evitando ciclos de congelación / descongelación (preparando alícuotas) y manteniéndolo a la temperatura adecuada.

Espero haberte ayudado. Para cualquier otra consulta o sugerencia, por favor no dudes en volvernos a contactar.

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