Recombinant
RabMAb

Recombinant Anti-Fatty Acid Synthase antibody [EPR7466] (Alexa Fluor® 647) (ab196853)

Rabbit recombinant monoclonal Fatty Acid Synthase antibody [EPR7466] conjugated to Alexa Fluor® 647. Validated in ICC/IF and tested in Mouse, Rat, Human.

Overview

  • Product name

    Anti-Fatty Acid Synthase antibody [EPR7466] (Alexa Fluor® 647)
    See all Fatty Acid Synthase primary antibodies
  • Description

    Rabbit monoclonal [EPR7466] to Fatty Acid Synthase (Alexa Fluor® 647)
  • Host species

    Rabbit
  • Conjugation

    Alexa Fluor® 647. Ex: 652nm, Em: 668nm
  • Tested applications

    Suitable for: ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Fatty Acid Synthase aa 2450 to the C-terminus (C terminal). The exact sequence is proprietary.

  • Positive control

    • ICC/IF: A549 cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

    This product is a recombinant rabbit monoclonal antibody.

Applications

Our Abpromise guarantee covers the use of ab196853 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/50.

This product gave a positive signal in A549 cells fixed with 100% methanol (5 min)

Target

  • Function

    Fatty acid synthetase catalyzes the formation of long-chain fatty acids from acetyl-CoA, malonyl-CoA and NADPH. This multifunctional protein has 7 catalytic activities and an acyl carrier protein.
  • Tissue specificity

    Ubiquitous. Prominent expression in brain, lung, and liver.
  • Sequence similarities

    Contains 1 acyl carrier domain.
  • Cellular localization

    Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links

  • Alternative names

    • [Acyl-carrier-protein] S acetyltransferase antibody
    • [Acyl-carrier-protein] S malonyltransferase antibody
    • 3-hydroxypalmitoyl-[acyl-carrier-protein] dehydratase antibody
    • 3-oxoacyl-[acyl-carrier-protein] reductase antibody
    • 3-oxoacyl-[acyl-carrier-protein] synthase antibody
    • Enoyl-[acyl-carrier-protein] reductase antibody
    • FAS antibody
    • FAS_HUMAN antibody
    • FASN antibody
    • Fatty acid synthase antibody
    • MGC14367 antibody
    • MGC15706 antibody
    • OA 519 antibody
    • Oleoyl-[acyl-carrier-protein] hydrolase antibody
    • SDR27X1 antibody
    • Short chain dehydrogenase/reductase family 27X member 1 antibody
    see all

Images

  • ab196853 staining Fatty Acid Synthase in A549 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab196853 at a 1/50 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at a 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

References

ab196853 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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