Validated using a knockout cell line
Recombinant
RabMAb

Recombinant Anti-Fatty Acid Synthase antibody [EPR7466] (HRP) (ab196854)

Overview

  • Product name

    Anti-Fatty Acid Synthase antibody [EPR7466] (HRP)
    See all Fatty Acid Synthase primary antibodies
  • Description

    Rabbit monoclonal [EPR7466] to Fatty Acid Synthase (HRP)
  • Host species

    Rabbit
  • Conjugation

    HRP
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human Fatty Acid Synthase aa 2450 to the C-terminus (C terminal). The exact sequence is proprietary.

  • Positive control

    • WB: HeLa, HEK293 and A549 whole cell lysates.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Applications

Our Abpromise guarantee covers the use of ab196854 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Detects a band of approximately 273 kDa (predicted molecular weight: 273 kDa).

Target

  • Function

    Fatty acid synthetase catalyzes the formation of long-chain fatty acids from acetyl-CoA, malonyl-CoA and NADPH. This multifunctional protein has 7 catalytic activities and an acyl carrier protein.
  • Tissue specificity

    Ubiquitous. Prominent expression in brain, lung, and liver.
  • Sequence similarities

    Contains 1 acyl carrier domain.
  • Cellular localization

    Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links

  • Alternative names

    • [Acyl-carrier-protein] S acetyltransferase antibody
    • [Acyl-carrier-protein] S malonyltransferase antibody
    • 3-hydroxypalmitoyl-[acyl-carrier-protein] dehydratase antibody
    • 3-oxoacyl-[acyl-carrier-protein] reductase antibody
    • 3-oxoacyl-[acyl-carrier-protein] synthase antibody
    • Enoyl-[acyl-carrier-protein] reductase antibody
    • FAS antibody
    • FAS_HUMAN antibody
    • FASN antibody
    • Fatty acid synthase antibody
    • MGC14367 antibody
    • MGC15706 antibody
    • OA 519 antibody
    • Oleoyl-[acyl-carrier-protein] hydrolase antibody
    • SDR27X1 antibody
    • Short chain dehydrogenase/reductase family 27X member 1 antibody
    see all

Images

  • All lanes : Anti-Fatty Acid Synthase antibody [EPR7466] (HRP) (ab196854) at 1/5000 dilution

    Lane 1 : Wild-type HAP1 whole cell lysate
    Lane 2 : FASN (Fatty Acid Synthase) knockout HAP1 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 273 kDa
    Observed band size: 260 kDa
    why is the actual band size different from the predicted?


    Exposure time: 1 minute


    ab196854 was shown to specifically react with Fatty Acid Synthase in wild-type HAP1 cells as signal was lost in FASN (Fatty Acid Synthase) knockout cells. Wild-type and FASN (Fatty Acid Synthase) knockout samples were subjected to SDS-PAGE. Ab196854 and ab184095 (Mouse monoclonal [mAbcam 9484] to GAPDH - Loading Control (Alexa Fluor® 680) loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/1000 dilution respectively. The loading control was imaged using the Licor Odyssey CLx prior to blots being developed with ECL technique.

  • All lanes : Anti-Fatty Acid Synthase antibody [EPR7466] (HRP) (ab196854) at 1/5000 dilution

    Lane 1 : HeLa whole cell lysate (ab150035)
    Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 3 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 273 kDa
    Observed band size: 273 kDa


    Exposure time: 8 minutes


    This blot was produced using a 3-8% Tris Acetate gel under the TA buffer system. The gel was run at 150V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab196854 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

References

This product has been referenced in:

  • Jeong SH  et al. Hippo-mediated suppression of IRS2/AKT signaling prevents hepatic steatosis and liver cancer. J Clin Invest 128:1010-1025 (2018). Read more (PubMed: 29400692) »
  • Wang Y  et al. Cannabidiol attenuates alcohol-induced liver steatosis, metabolic dysregulation, inflammation and neutrophil-mediated injury. Sci Rep 7:12064 (2017). WB . Read more (PubMed: 28935932) »
See all 2 Publications for this product

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