Recombinant Anti-FBP1 + FBP2 antibody [EPR4619] (ab109020)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4619] to FBP1 + FBP2
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-FBP1 + FBP2 antibody [EPR4619] -
Description
Rabbit monoclonal [EPR4619] to FBP1 + FBP2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: ICC/IF -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HL60 lysate treated with vitamin D3, MCF7 and Human fetal liver lysates; Human kidney and liver tissues.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR4619 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab109020 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 37 kDa.
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Heat up to 98 °C, below boiling, and then let cool for 10-20 min. |
Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 37 kDa. |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Heat up to 98 °C, below boiling, and then let cool for 10-20 min. |
Target
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Cellular localization
FBP2: Cell junction. Cytoplasm. Nucleus. Cytoplasm, myofibril, sarcomere, Z line. In neonatal cardiomyocytes, distributed throughout the cytosol, accumulating in the intercalated disks which occur at the Z line of cardiomyocytes and connect adjacent cells, and also located in the nucleus; dissociates from the Z line following an increase in cytosolic Ca(2+) concentration (By similarity). In muscle precursor cells, localizes predominantly to the nucleus and to a lesser extent to the cytoplasm at the proliferative phase, while mainly localizing to the cytoplasm at the differentiation phase (By similarity). Colocalizes with ALDOA and alpha-actinin on both sides of the Z line of skeletal muscle; dissociates rapidly from the Z line following an increase in cytosolic Ca(2+) concentration. -
Database links
- Entrez Gene: 2203 Human
- Entrez Gene: 8789 Human
- Entrez Gene: 14120 Mouse
- Entrez Gene: 14121 Mouse
- Entrez Gene: 114508 Rat
- Entrez Gene: 24362 Rat
- Omim: 603027 Human
- Omim: 611570 Human
see all
Images
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ab109020 at 1:500 staining Anti-FBP1 + FBP2 antibody in mouse kidney tissue by immunohistochemistry (FFPE). Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling FBP1 with ab109020 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP). Counter stained with hematoxylin.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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All lanes : Anti-FBP1 + FBP2 antibody [EPR4619] (ab109020) at 1/10000 dilution
Lane 1 : C-Myc/DDK-tagged human FBP1 full length recombinant protein
Lane 2 : C-Myc/DDK-tagged human FBP2 full length recombinant protein
Lysates/proteins at 0.01 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 37 kDa
Observed band size: 37 kDa
Exposure time: 180 secondsBlocking/Diluting buffer and concentration: 5% NFDM/TBST
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All lanes : Anti-FBP1 + FBP2 antibody [EPR4619] (ab109020) at 1/10000 dilution (purified)
Lane 1 : Mouse liver
Lane 2 : Rat liver
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 37 kDa
Observed band size: 37 kDaBlocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
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All lanes : Anti-FBP1 + FBP2 antibody [EPR4619] (ab109020) at 1/2000 dilution (purified)
Lane 1 : MCF7 cell lysate
Lane 2 : HL-60 cell lysate treated with vitamin D3
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP goat anti-rabbit (H+L) at 1/2000 dilution
Predicted band size: 37 kDa
Observed band size: 37 kDaBlocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
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ab109020 at 1:500 staining Anti-FBP1 + FBP2 antibody in human liver tissue by immunohistochemistry (FFPE). Immunohistochemical analysis of paraffin-embedded human liver tissue labeling FBP1 with ab109020 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP). Counter stained with hematoxylin.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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All lanes : Anti-FBP1 + FBP2 antibody [EPR4619] (ab109020) at 1/1000 dilution (unpurified)
Lane 1 : MCF7 cell lysate
Lane 2 : Human fetal liver tissue lysate
Lane 3 : HL60 cell lysate, treated with vitamin D3
Lysates/proteins at 10 µg per lane.
Predicted band size: 37 kDaBlocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
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Unpurified ab109020 at 1/50 dilution staining FBP1 + FBP2 in Human Kidney by immunohistochemistry, paraffin-embedded tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Unpurified ab109020 at 1/50 dilution staining FBP1 + FBP2 in Human liver by immunohistochemistry, paraffin-embedded tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (12)
ab109020 has been referenced in 12 publications.
- Sadiku P et al. Neutrophils Fuel Effective Immune Responses through Gluconeogenesis and Glycogenesis. Cell Metab 33:411-423.e4 (2021). PubMed: 33306983
- Fan Z et al. LOXL2 upregulates hypoxia-inducible factor-1a signaling through Snail-FBP1 axis in hepatocellular carcinoma cells. Oncol Rep 43:1641-1649 (2020). PubMed: 32323822
- Becker LM et al. Epigenetic Reprogramming of Cancer-Associated Fibroblasts Deregulates Glucose Metabolism and Facilitates Progression of Breast Cancer. Cell Rep 31:107701 (2020). PubMed: 32492417
- Aljaylani A et al. Acid Loading Unmasks Glucose Homeostatic Instability in Proximal-Tubule-Targeted Insulin/Insulin-Like-Growth-Factor-1 Receptor Dual Knockout Mice. Cell Physiol Biochem 54:682-695 (2020). PubMed: 32678535
- Selmi-Ruby S et al. In Vivo Characterization of the Toxicological Properties of DPhP, One of the Main Degradation Products of Aryl Phosphate Esters. Environ Health Perspect 128:127006 (2020). PubMed: 33296241