Product nameAnti-FBP1 antibody [EPR4620]
See all FBP1 primary antibodies
DescriptionRabbit monoclonal [EPR4620] to FBP1
Tested applicationsSuitable for: ICC/IF, WB, IP, ICCmore details
Unsuitable for: Flow Cyt or IHC-P
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human FBP1 aa 300-400. The exact sequence is proprietary.
- Human stomach, Human fetal liver, and MCF7 lysates IF/ICC: HeLa cell line
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
Our Abpromise guarantee covers the use of ab109732 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 10 µg/ml.|
|WB||1/1000 - 1/10000. Detects a band of approximately 36 kDa (predicted molecular weight: 37 kDa).|
|IP||1/10 - 1/100.|
|ICC||1/100 - 1/250.|
PathwayCarbohydrate biosynthesis; gluconeogenesis.
Involvement in diseaseDefects in FBP1 are the cause of fructose-1,6-bisphosphatase deficiency (FBPD) [MIM:229700]. FBPD is inherited as an autosomal recessive disorder mainly in the liver and causes life-threatening episodes of hypoglycemia and metabolic acidosis (lactacidemia) in newborn infants or young children.
Sequence similaritiesBelongs to the FBPase class 1 family.
- Information by UniProt
- 6-bisphosphatase 1 antibody
- 6-bisphosphate 1-phosphohydrolase 1 antibody
- D fructose 1 6 bisphosphate 1 phosphohydrolase 1 antibody
ICC/IF image of ab109732 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab109732, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
All lanes : Anti-FBP1 antibody [EPR4620] (ab109732) at 1/1000 dilution
Lane 1 : Human stomach lysate
Lane 2 : Human fetal liver lysate
Lane 3 : MCF7 lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 37 kDa
This product has been referenced in:
- Yan Y et al. Dual inhibition of AKT-mTOR and AR signaling by targeting HDAC3 in PTEN- or SPOP-mutated prostate cancer. EMBO Mol Med 10:N/A (2018). Read more (PubMed: 29523594) »
- Guo B et al. Antagonism of PPAR-? signaling expands human hematopoietic stem and progenitor cells by enhancing glycolysis. Nat Med 24:360-367 (2018). ICC/IF ; Human . Read more (PubMed: 29377004) »