Overview

  • Product name
    Anti-FBP1 antibody [EPR4620]
    See all FBP1 primary antibodies
  • Description
    Rabbit monoclonal [EPR4620] to FBP1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, WB, ICC, Flow Cytmore details
    Unsuitable for: IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human FBP1 aa 300-400. The exact sequence is proprietary.

  • Positive control
    • WB: Human stomach, Human fetal liver, Human kidney, Mouse liver, Mouse kidney, Rat liver, and MCF7 lysates IF/ICC: HeLa cell line FC: MCF7 cells
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab109732 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 10 µg/ml.
WB 1/1000 - 1/10000. Detects a band of approximately 36 kDa (predicted molecular weight: 37 kDa).
ICC 1/50.

For unpurified use at 1/100 - 1/250.

Flow Cyt 1/400.
  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Pathway
      Carbohydrate biosynthesis; gluconeogenesis.
    • Involvement in disease
      Defects in FBP1 are the cause of fructose-1,6-bisphosphatase deficiency (FBPD) [MIM:229700]. FBPD is inherited as an autosomal recessive disorder mainly in the liver and causes life-threatening episodes of hypoglycemia and metabolic acidosis (lactacidemia) in newborn infants or young children.
    • Sequence similarities
      Belongs to the FBPase class 1 family.
    • Information by UniProt
    • Database links
    • Alternative names
      • 6-bisphosphatase 1 antibody
      • 6-bisphosphate 1-phosphohydrolase 1 antibody
      • D fructose 1 6 bisphosphate 1 phosphohydrolase 1 antibody
      • D-fructose-1 antibody
      • EC 3.1.3.11 antibody
      • F16P1_HUMAN antibody
      • FBP antibody
      • FBP 1 antibody
      • FBP1 antibody
      • FBPase 1 antibody
      • Fructose 1 6 bisphosphatase 1 antibody
      • Fructose bisphosphatase 1 antibody
      • Fructose-1 antibody
      • Growth inhibiting protein 17 antibody
      • Liver fructose bisphosphatase antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling FBP1 with purified ab109732 at 1:50 dilution (7.9 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
    • All lanes : Anti-FBP1 antibody [EPR4620] (ab109732) at 1/1000 dilution (Purified)

      Lane 1 : Human kidney lysates
      Lane 2 : Mouse liver lysates
      Lane 3 : Mouse kidney lysates
      Lane 4 : Rat liver lysates

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 37 kDa
      Observed band size: 36 kDa
      why is the actual band size different from the predicted?

    • Anti-FBP1 antibody [EPR4620] (ab109732) at 1/50000 dilution + MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysates at 15 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 37 kDa
      Observed band size: 36 kDa why is the actual band size different from the predicted?

    • Flow Cytometry analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling FBP1 with purified ab109732 at 1:400 dilution (1 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
    • ICC/IF image of ab109732 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab109732, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    • All lanes : Anti-FBP1 antibody [EPR4620] (ab109732) at 1/1000 dilution

      Lane 1 : Human stomach lysate
      Lane 2 : Human fetal liver lysate
      Lane 3 : MCF7 lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 37 kDa

    References

    This product has been referenced in:
    • Yan Y  et al. Dual inhibition of AKT-mTOR and AR signaling by targeting HDAC3 in PTEN- or SPOP-mutated prostate cancer. EMBO Mol Med 10:N/A (2018). Read more (PubMed: 29523594) »
    • Guo B  et al. Antagonism of PPAR-? signaling expands human hematopoietic stem and progenitor cells by enhancing glycolysis. Nat Med 24:360-367 (2018). ICC/IF ; Human . Read more (PubMed: 29377004) »
    See all 7 Publications for this product

    Customer reviews and Q&As

    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Liver)
    Gel Running Conditions
    Non-reduced Denaturing (12%)
    Loading amount
    30 µg
    Specification
    Liver
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C

    Abcam user community

    Verified customer

    Submitted Jun 22 2017

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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