• Product name
    Anti-FBP1 antibody [EPR4620]
    See all FBP1 primary antibodies
  • Description
    Rabbit monoclonal [EPR4620] to FBP1
  • Host species
  • Tested applications
    Suitable for: ICC/IF, WB, IP, ICCmore details
    Unsuitable for: Flow Cyt or IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human FBP1 aa 300-400. The exact sequence is proprietary.

  • Positive control
    • Human stomach, Human fetal liver, and MCF7 lysates IF/ICC: HeLa cell line
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.



Our Abpromise guarantee covers the use of ab109732 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 10 µg/ml.
WB 1/1000 - 1/10000. Detects a band of approximately 36 kDa (predicted molecular weight: 37 kDa).
IP 1/10 - 1/100.
ICC 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt or IHC-P.
  • Target

    • Pathway
      Carbohydrate biosynthesis; gluconeogenesis.
    • Involvement in disease
      Defects in FBP1 are the cause of fructose-1,6-bisphosphatase deficiency (FBPD) [MIM:229700]. FBPD is inherited as an autosomal recessive disorder mainly in the liver and causes life-threatening episodes of hypoglycemia and metabolic acidosis (lactacidemia) in newborn infants or young children.
    • Sequence similarities
      Belongs to the FBPase class 1 family.
    • Information by UniProt
    • Database links
    • Alternative names
      • 6-bisphosphatase 1 antibody
      • 6-bisphosphate 1-phosphohydrolase 1 antibody
      • D fructose 1 6 bisphosphate 1 phosphohydrolase 1 antibody
      • D-fructose-1 antibody
      • EC antibody
      • F16P1_HUMAN antibody
      • FBP antibody
      • FBP 1 antibody
      • FBP1 antibody
      • FBPase 1 antibody
      • Fructose 1 6 bisphosphatase 1 antibody
      • Fructose bisphosphatase 1 antibody
      • Fructose-1 antibody
      • Growth inhibiting protein 17 antibody
      • Liver fructose bisphosphatase antibody
      see all


    • ICC/IF image of ab109732 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab109732, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    • All lanes : Anti-FBP1 antibody [EPR4620] (ab109732) at 1/1000 dilution

      Lane 1 : Human stomach lysate
      Lane 2 : Human fetal liver lysate
      Lane 3 : MCF7 lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 37 kDa


    This product has been referenced in:
    • Yan Y  et al. Dual inhibition of AKT-mTOR and AR signaling by targeting HDAC3 in PTEN- or SPOP-mutated prostate cancer. EMBO Mol Med 10:N/A (2018). Read more (PubMed: 29523594) »
    • Guo B  et al. Antagonism of PPAR-? signaling expands human hematopoietic stem and progenitor cells by enhancing glycolysis. Nat Med 24:360-367 (2018). ICC/IF ; Human . Read more (PubMed: 29377004) »
    See all 7 Publications for this product

    Customer reviews and Q&As

    Western blot
    Mouse Tissue lysate - whole (Liver)
    Gel Running Conditions
    Non-reduced Denaturing (12%)
    Loading amount
    30 µg
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C

    Abcam user community

    Verified customer

    Submitted Jun 22 2017


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