Recombinant Anti-FBP1 antibody [EPR4620] (ab109732)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4620] to FBP1
- Suitable for: Flow Cyt (Intra), ICC/IF, WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-FBP1 antibody [EPR4620]
See all FBP1 primary antibodies -
Description
Rabbit monoclonal [EPR4620] to FBP1 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), ICC/IF, WBmore details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human stomach, Human fetal liver, Human kidney, Mouse liver, Mouse kidney, Rat liver, and MCF7 lysates and DDDK-tagged FBP1 human full-length recombinant protein. ICC/IF: HeLa cell line Flow Cyt (intra): MCF7 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR4620 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab109732 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
1/400.
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ICC/IF |
Use a concentration of 10 µg/ml.
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WB | (1) |
1/1000 - 1/10000. Detects a band of approximately 36 kDa (predicted molecular weight: 37 kDa).
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Notes |
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Flow Cyt (Intra)
1/400. |
ICC/IF
Use a concentration of 10 µg/ml. |
WB
1/1000 - 1/10000. Detects a band of approximately 36 kDa (predicted molecular weight: 37 kDa). |
Target
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Pathway
Carbohydrate biosynthesis; gluconeogenesis. -
Involvement in disease
Defects in FBP1 are the cause of fructose-1,6-bisphosphatase deficiency (FBPD) [MIM:229700]. FBPD is inherited as an autosomal recessive disorder mainly in the liver and causes life-threatening episodes of hypoglycemia and metabolic acidosis (lactacidemia) in newborn infants or young children. -
Sequence similarities
Belongs to the FBPase class 1 family. - Information by UniProt
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Database links
- Entrez Gene: 2203 Human
- Entrez Gene: 14121 Mouse
- Entrez Gene: 24362 Rat
- Omim: 611570 Human
- SwissProt: P09467 Human
- SwissProt: Q9QXD6 Mouse
- SwissProt: P19112 Rat
- Unigene: 494496 Human
see all -
Alternative names
- 6-bisphosphatase 1 antibody
- 6-bisphosphate 1-phosphohydrolase 1 antibody
- D fructose 1 6 bisphosphate 1 phosphohydrolase 1 antibody
see all
Images
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All lanes : Anti-FBP1 antibody [EPR4620] (ab109732) at 1/1000 dilution
Lane 1 : DDDK-tagged FBP1 human full-length recombinant protein, 15 ng
Lane 2 : DDDK-tagged FBP12 human full-length recombinant protein, 15 ng
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 37 kDa
Observed band size: 37 kDa
Exposure time: 5 secondsBlocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
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Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling FBP1 with purified ab109732 at 1:50 dilution (7.9 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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All lanes : Anti-FBP1 antibody [EPR4620] (ab109732) at 1/1000 dilution (Purified)
Lane 1 : Human kidney lysates
Lane 2 : Mouse liver lysates
Lane 3 : Mouse kidney lysates
Lane 4 : Rat liver lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 37 kDa
Observed band size: 36 kDa why is the actual band size different from the predicted? -
Anti-FBP1 antibody [EPR4620] (ab109732) at 1/50000 dilution + MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysates at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 37 kDa
Observed band size: 36 kDa why is the actual band size different from the predicted? -
Intracellular Flow Cytometry analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling FBP1 with purified ab109732 at 1/400 dilution (1 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
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ICC/IF image of ab109732 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab109732, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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All lanes : Anti-FBP1 antibody [EPR4620] (ab109732) at 1/1000 dilution
Lane 1 : Human stomach lysate
Lane 2 : Human fetal liver lysate
Lane 3 : MCF7 lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 37 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (14)
ab109732 has been referenced in 14 publications.
- Hu J et al. Fructose-1,6-bisphosphatase aggravates oxidative stress-induced apoptosis in asthma by suppressing the Nrf2 pathway. J Cell Mol Med 25:5001-5014 (2021). PubMed: 33960626
- Chen Z et al. Andrographolide inhibits non-small cell lung cancer cell proliferation through the activation of the mitochondrial apoptosis pathway and by reprogramming host glucose metabolism. Ann Transl Med 9:1701 (2021). PubMed: 34988210
- Han F et al. Gastric cancer mesenchymal stem cells inhibit natural killer cell function by up-regulating FBP1. Cent Eur J Immunol 46:427-437 (2021). PubMed: 35125940
- Yang C et al. USP44 suppresses pancreatic cancer progression and overcomes gemcitabine resistance by deubiquitinating FBP1. Am J Cancer Res 9:1722-1733 (2019). PubMed: 31497353
- Kanai S et al. Phosphofructokinase-1 and fructose bisphosphatase-1 in canine liver and kidney. J Vet Med Sci 81:1515-1521 (2019). PubMed: 31474665