Anti-FBP17 antibody (ab100918)
Key features and details
- Rabbit polyclonal to FBP17
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-FBP17 antibody
See all FBP17 primary antibodies -
Description
Rabbit polyclonal to FBP17 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rat, Horse, Chicken, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Orangutan, Platypus -
Immunogen
Other Immunogen Type within Human FBP17 aa 350-400. The exact sequence is proprietary.
Database link: NP_055848.1 -
Positive control
- HeLa, 293T lysates
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab100918 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 71 kDa.
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IP |
Use at 5-15 µg/mg of lysate.
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Notes |
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WB
1/2000 - 1/10000. Predicted molecular weight: 71 kDa. |
IP
Use at 5-15 µg/mg of lysate. |
Target
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Function
May act as a link between RND2 signaling and regulation of the actin cytoskeleton (By similarity). Required to coordinate membrane tubulation with reorganization of the actin cytoskeleton during endocytosis. Binds to lipids such as phosphatidylinositol 4,5-bisphosphate and phosphatidylserine and promotes membrane invagination and the formation of tubules. Also enhances actin polymerization via the recruitment of WASL/N-WASP, which in turn activates the Arp2/3 complex. Actin polymerization may promote the fission of membrane tubules to form endocytic vesicles. May be required for the lysosomal retention of FASLG/FASL. -
Tissue specificity
Very highly expressed in the epithelial cells of the gastrointestinal tract, respiratory, reproductive and urinary systems. Also highly expressed in brown adipose tissue, cardiomyocytes, enteric ganglia and glucagon producing cells of the pancreas. Expressed in germ cells of the testis and all regions of the brain. -
Involvement in disease
Note=A chromosomal aberration involving FNBP1 is found in acute leukemias. Translocation t(9;11)(q34;q23) with MLL. The relatively low incidence of the MLL-FNBP1 fusion protein in acute leukemia may reflect the marginal capacity of this fusion protein to induce cellular transformation. -
Sequence similarities
Belongs to the FNBP1 family.
Contains 1 FCH domain.
Contains 1 REM (Hr1) repeat.
Contains 1 SH3 domain. -
Cellular localization
Cytoplasm. Cytoplasm > cytoskeleton. Cytoplasm > cell cortex. Lysosome. Cytoplasmic vesicle. Cell membrane. Enriched in cortical regions coincident with F-actin. Also localizes to endocytic vesicles and lysosomes. - Information by UniProt
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Database links
- Entrez Gene: 23048 Human
- Entrez Gene: 192348 Rat
- Omim: 606191 Human
- SwissProt: Q96RU3 Human
- SwissProt: Q8R511 Rat
- Unigene: 189409 Human
- Unigene: 20445 Rat
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Alternative names
- FBP1 antibody
- FBP17 antibody
- Fnbp1 antibody
see all
Images
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All lanes : Anti-FBP17 antibody (ab100918) at 0.1 µg/ml
Lane 1 : HeLa Whole Cell Lysate at 50 µg
Lane 2 : HeLa Whole Cell Lysate at 15 µg
Lane 3 : HeLa Whole Cell Lysate at 5 µg
Lane 4 : 293T Whole Cell Lysate at 50 µg
Predicted band size: 71 kDa
Exposure time: 3 minutes -
ab100918 at 1 µg/ml staining FBP17 in HeLa cell lysate immunoprecipitated using ab100918 at 10 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Exposure time: 30 seconds
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab100918 has been referenced in 1 publication.
- Cao R et al. Silencing long intergenic non-protein coding RNA 00987 inhibits proliferation, migration, and invasion of osteosarcoma cells by sponging miR-376a-5p to regulate FNBP1 expression. Discov Oncol 12:18 (2021). PubMed: 35201476