
Recombinant Anti-FBP2 antibody [EPR7436] (ab131253)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Anti-FBP2 antibody [EPR7436]
See all FBP2 primary antibodies -
Description
Rabbit monoclonal [EPR7436] to FBP2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human FBP2 aa 300-400 (C terminal). The exact sequence is proprietary.
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Positive control
- Human skeletal muscle, Rat muscle and Mouse muscle lysates; Human muscle tissue.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 2.90 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR7436 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab131253 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | 1/1000 - 1/10000. Detects a band of approximately 34 kDa (predicted molecular weight: 37 kDa). | |
IHC-P | 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Catalyzes the hydrolysis of fructose 1,6-bisphosphate to fructose 6-phosphate in the presence of divalent cations and probably participates in glycogen synthesis from carbohydrate precursors, such as lactate. -
Tissue specificity
Expressed in skeletal muscle (at protein level). -
Pathway
Carbohydrate biosynthesis; gluconeogenesis. -
Sequence similarities
Belongs to the FBPase class 1 family. -
Cellular localization
Cell junction. Cytoplasm. Nucleus. Cytoplasm, myofibril, sarcomere, Z line. In neonatal cardiomyocytes, distributed throughout the cytosol, accumulating in the intercalated disks which occur at the Z line of cardiomyocytes and connect adjacent cells, and also located in the nucleus; dissociates from the Z line following an increase in cytosolic Ca(2+) concentration (By similarity). In muscle precursor cells, localizes predominantly to the nucleus and to a lesser extent to the cytoplasm at the proliferative phase, while mainly localizing to the cytoplasm at the differentiation phase (By similarity). Colocalizes with ALDOA and alpha-actinin on both sides of the Z line of skeletal muscle; dissociates rapidly from the Z line following an increase in cytosolic Ca(2+) concentration. - Information by UniProt
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Database links
- Entrez Gene: 8789 Human
- Entrez Gene: 14120 Mouse
- Entrez Gene: 114508 Rat
- Omim: 603027 Human
- SwissProt: O00757 Human
- SwissProt: P70695 Mouse
- SwissProt: Q9Z1N1 Rat
- Unigene: 61255 Human
see all -
Alternative names
- 6-bisphosphatase isozyme 2 antibody
- 6-bisphosphate 1-phosphohydrolase 2 antibody
- D fructose 1 6 bisphosphate 1 phosphohydrolase 2 antibody
see all
Images
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All lanes : Anti-FBP2 antibody [EPR7436] (ab131253) at 1/1000 dilution
Lane 1 : Human skeletal muscle lysate
Lane 2 : Rat muscle lysate
Lane 3 : Mouse muscle lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled Goat anti Rabbit IgG at 1/2000 dilution
Predicted band size: 37 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FBP2 antibody [EPR7436] (ab131253)
Immunohistochemical analysis of paraffin embedded Human muscle tissue labelling FBP2 with ab131253 antibody at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Datasheets and documents
References
This product has been referenced in:
- Wang C et al. Long-term supranutritional supplementation with selenate decreases hyperglycemia and promotes fatty liver degeneration by inducing hyperinsulinemia in diabetic db/db mice. PLoS One 9:e101315 (2014). WB . Read more (PubMed: 24983750) »