RecombinantRabMAb

Recombinant Anti-FBP2 antibody [EPR7436] (ab131253)

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Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7436] to FBP2
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-FBP2 antibody [EPR7436]
    See all FBP2 primary antibodies

  • Description

    Rabbit monoclonal [EPR7436] to FBP2

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human FBP2 aa 300-400 (C terminal). The exact sequence is proprietary.

  • Positive control

    • Human skeletal muscle, Rat muscle and Mouse muscle lysates; Human muscle tissue.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab131253 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 34 kDa (predicted molecular weight: 37 kDa).
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for IP.

    • Target

    • Function

      Catalyzes the hydrolysis of fructose 1,6-bisphosphate to fructose 6-phosphate in the presence of divalent cations and probably participates in glycogen synthesis from carbohydrate precursors, such as lactate.

    • Tissue specificity

      Expressed in skeletal muscle (at protein level).

    • Pathway

      Carbohydrate biosynthesis; gluconeogenesis.

    • Sequence similarities

      Belongs to the FBPase class 1 family.

    • Cellular localization

      Cell junction. Cytoplasm. Nucleus. Cytoplasm, myofibril, sarcomere, Z line. In neonatal cardiomyocytes, distributed throughout the cytosol, accumulating in the intercalated disks which occur at the Z line of cardiomyocytes and connect adjacent cells, and also located in the nucleus; dissociates from the Z line following an increase in cytosolic Ca(2+) concentration (By similarity). In muscle precursor cells, localizes predominantly to the nucleus and to a lesser extent to the cytoplasm at the proliferative phase, while mainly localizing to the cytoplasm at the differentiation phase (By similarity). Colocalizes with ALDOA and alpha-actinin on both sides of the Z line of skeletal muscle; dissociates rapidly from the Z line following an increase in cytosolic Ca(2+) concentration.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • 6-bisphosphatase isozyme 2 antibody
      • 6-bisphosphate 1-phosphohydrolase 2 antibody
      • D fructose 1 6 bisphosphate 1 phosphohydrolase 2 antibody
      • D-fructose-1 antibody
      • F16P2_HUMAN antibody
      • FBP 2 antibody
      • fbp2 antibody
      • FBPase 2 antibody
      • FBPase antibody
      • fructose 1 6 bisphosphatase 2 antibody
      • Fructose 1 6 bisphosphatase isozyme 2 antibody
      • Fructose-1 antibody
      • Hexosediphosphatase antibody
      • MGC142192 antibody
      • Muscle FBPase antibody
      • Muscle fructose bisphosphatase antibody
      see all

    Images

    • Western blot - Anti-FBP2 antibody [EPR7436] (ab131253)
      Western blot - Anti-FBP2 antibody [EPR7436] (ab131253)
      All lanes : Anti-FBP2 antibody [EPR7436] (ab131253) at 1/1000 dilution

      Lane 1 : Human skeletal muscle lysate
      Lane 2 : Rat muscle lysate
      Lane 3 : Mouse muscle lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled Goat anti Rabbit IgG at 1/2000 dilution

      Predicted band size: 37 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FBP2 antibody [EPR7436] (ab131253)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FBP2 antibody [EPR7436] (ab131253)

      Immunohistochemical analysis of paraffin embedded Human muscle tissue labelling FBP2 with ab131253 antibody at 1/50 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • OI-RD Scanning - Anti-FBP2 antibody [EPR7436] (ab131253)
      OI-RD Scanning - Anti-FBP2 antibody [EPR7436] (ab131253)
      Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD
    • Anti-FBP2 antibody [EPR7436] (ab131253)
      Anti-FBP2 antibody [EPR7436] (ab131253)

    References (1)

    Publishing research using ab131253? Please let us know so that we can cite the reference in this datasheet.

    ab131253 has been referenced in 1 publication.

    • Wang C  et al. Long-term supranutritional supplementation with selenate decreases hyperglycemia and promotes fatty liver degeneration by inducing hyperinsulinemia in diabetic db/db mice. PLoS One 9:e101315 (2014). WB . PubMed: 24983750

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