Overview

  • Product name

    Anti-FBP2 antibody [EPR7436]
    See all FBP2 primary antibodies
  • Description

    Rabbit monoclonal [EPR7436] to FBP2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human FBP2 aa 300-400 (C terminal). The exact sequence is proprietary.

  • Positive control

    • Human skeletal muscle, Rat muscle and Mouse muscle lysates; Human muscle tissue.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab131253 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 34 kDa (predicted molecular weight: 37 kDa).
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      Catalyzes the hydrolysis of fructose 1,6-bisphosphate to fructose 6-phosphate in the presence of divalent cations and probably participates in glycogen synthesis from carbohydrate precursors, such as lactate.
    • Tissue specificity

      Expressed in skeletal muscle (at protein level).
    • Pathway

      Carbohydrate biosynthesis; gluconeogenesis.
    • Sequence similarities

      Belongs to the FBPase class 1 family.
    • Cellular localization

      Cell junction. Cytoplasm. Nucleus. Cytoplasm, myofibril, sarcomere, Z line. In neonatal cardiomyocytes, distributed throughout the cytosol, accumulating in the intercalated disks which occur at the Z line of cardiomyocytes and connect adjacent cells, and also located in the nucleus; dissociates from the Z line following an increase in cytosolic Ca(2+) concentration (By similarity). In muscle precursor cells, localizes predominantly to the nucleus and to a lesser extent to the cytoplasm at the proliferative phase, while mainly localizing to the cytoplasm at the differentiation phase (By similarity). Colocalizes with ALDOA and alpha-actinin on both sides of the Z line of skeletal muscle; dissociates rapidly from the Z line following an increase in cytosolic Ca(2+) concentration.
    • Information by UniProt
    • Database links

    • Alternative names

      • 6-bisphosphatase isozyme 2 antibody
      • 6-bisphosphate 1-phosphohydrolase 2 antibody
      • D fructose 1 6 bisphosphate 1 phosphohydrolase 2 antibody
      • D-fructose-1 antibody
      • F16P2_HUMAN antibody
      • FBP 2 antibody
      • fbp2 antibody
      • FBPase 2 antibody
      • FBPase antibody
      • fructose 1 6 bisphosphatase 2 antibody
      • Fructose 1 6 bisphosphatase isozyme 2 antibody
      • Fructose-1 antibody
      • Hexosediphosphatase antibody
      • MGC142192 antibody
      • Muscle FBPase antibody
      • Muscle fructose bisphosphatase antibody
      see all

    Images

    • All lanes : Anti-FBP2 antibody [EPR7436] (ab131253) at 1/1000 dilution

      Lane 1 : Human skeletal muscle lysate
      Lane 2 : Rat muscle lysate
      Lane 3 : Mouse muscle lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled Goat anti Rabbit IgG at 1/2000 dilution

      Predicted band size: 37 kDa

    • Immunohistochemical analysis of paraffin embedded Human muscle tissue labelling FBP2 with ab131253 antibody at 1/50 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:

    • Wang C  et al. Long-term supranutritional supplementation with selenate decreases hyperglycemia and promotes fatty liver degeneration by inducing hyperinsulinemia in diabetic db/db mice. PLoS One 9:e101315 (2014). WB . Read more (PubMed: 24983750) »
    See 1 Publication for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab131253.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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