Product nameAnti-FBW2 antibody
See all FBW2 primary antibodies
DescriptionRabbit polyclonal to FBW2
Tested applicationsSuitable for: WB, ELISAmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
- Jurkat whole cell lysate (ab7899).
This product was previously labelled as FbxW2
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab83467 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 52 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|ELISA||Use at an assay dependent concentration.
Titre using peptide based assay: 1/1562500.
FunctionSubstrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex.
Sequence similaritiesContains 1 F-box domain.
Contains 4 WD repeats.
- Information by UniProt
- F box and WD 40 domain containing protein 2 antibody
- F box and WD repeat domain containing 2 antibody
- F box and WD40 domain protein 2 antibody
Anti-FBW2 antibody (ab83467) at 1 µg/ml (in 5% skim milk / PBS buffer) + Jurkat cell lysate at 10 µg
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 52 kDa
Observed band size: 52 kDa
Additional bands at: 34 kDa. We are unsure as to the identity of these extra bands.
Gel concentration: 12%
This product has been referenced in:
- Yang F et al. FBXW2 suppresses migration and invasion of lung cancer cells via promoting ß-catenin ubiquitylation and degradation. Nat Commun 10:1382 (2019). Read more (PubMed: 30918250) »
- Xu J et al. The ß-TrCP-FBXW2-SKP2 axis regulates lung cancer cell growth with FBXW2 acting as a tumour suppressor. Nat Commun 8:14002 (2017). IHC . Read more (PubMed: 28090088) »