Key features and details
- Mouse polyclonal to Fbx32
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Fbx32 antibody
See all Fbx32 primary antibodies
DescriptionMouse polyclonal to Fbx32
SpecificityThis antibody is reactive against mammalian transfected lysate.
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Full length Fbx32 protein (Human)
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.4
Constituent: 2.68% PBS
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab67866 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 36 kDa (predicted molecular weight: 42 kDa).|
FunctionSubstrate recognition component of a (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Probably recognizes and binds to phosphorylated target proteins during skeletal muscle atrophy. Recognizes TERF1.
Tissue specificitySpecifically expressed in cardiac and skeletal muscle.
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesContains 1 F-box domain.
- Information by UniProt
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All lanes : Anti-Fbx32 antibody (ab67866) at 1/500 dilution
Lane 1 : Fbx32 transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size: 42 kDa
Observed band size: 36 kDa why is the actual band size different from the predicted?
ab67866 has been referenced in 3 publications.
- Calvo AC et al. Collagen XIX Alpha 1 Improves Prognosis in Amyotrophic Lateral Sclerosis. Aging Dis 10:278-292 (2019). PubMed: 31011479
- MacNeil LG et al. The order of exercise during concurrent training for rehabilitation does not alter acute genetic expression, mitochondrial enzyme activity or improvements in muscle function. PLoS One 9:e109189 (2014). WB ; Human . PubMed: 25289940
- Chou JL et al. Promoter hypermethylation of FBXO32, a novel TGF-beta/SMAD4 target gene and tumor suppressor, is associated with poor prognosis in human ovarian cancer. Lab Invest 90:414-25 (2010). IHC-P ; Human . PubMed: 20065949