Key features and details
- Mouse polyclonal to Fbx32
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Fbx32 antibody
See all Fbx32 primary antibodies
DescriptionMouse polyclonal to Fbx32
SpecificityThis antibody is reactive against mammalian transfected lysate.
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Full length Fbx32 protein (Human)
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.20
Constituent: 2.68% PBS
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab67866 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 36 kDa (predicted molecular weight: 42 kDa).|
FunctionSubstrate recognition component of a (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Probably recognizes and binds to phosphorylated target proteins during skeletal muscle atrophy. Recognizes TERF1.
Tissue specificitySpecifically expressed in cardiac and skeletal muscle.
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesContains 1 F-box domain.
- Information by UniProt
- 4833442G10Rik antibody
- AI430017 antibody
- Atrogin 1 antibody
All lanes : Anti-Fbx32 antibody (ab67866) at 1/500 dilution
Lane 1 : Fbx32 transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size: 42 kDa
Observed band size: 36 kDa why is the actual band size different from the predicted?
ab67866 has been referenced in 3 publications.
- Calvo AC et al. Collagen XIX Alpha 1 Improves Prognosis in Amyotrophic Lateral Sclerosis. Aging Dis 10:278-292 (2019). PubMed: 31011479
- MacNeil LG et al. The order of exercise during concurrent training for rehabilitation does not alter acute genetic expression, mitochondrial enzyme activity or improvements in muscle function. PLoS One 9:e109189 (2014). WB ; Human . PubMed: 25289940
- Chou JL et al. Promoter hypermethylation of FBXO32, a novel TGF-beta/SMAD4 target gene and tumor suppressor, is associated with poor prognosis in human ovarian cancer. Lab Invest 90:414-25 (2010). IHC-P ; Human . PubMed: 20065949