Anti-Fbx32 antibody (ab74023)
Key features and details
- Rabbit polyclonal to Fbx32
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Fbx32 antibody
See all Fbx32 primary antibodies -
Description
Rabbit polyclonal to Fbx32 -
Host species
Rabbit -
Specificity
This antibody is only batch tested in WB and therefore we can only guarantee it for this application. Customers have successfully used this antibody in IHC and ICC but we have not tested it's performance in these applications. If you would like an antibody that is tested in ICC, we would recommend our recombinant antibody ab168372. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Chicken, Cow, Pig, Zebrafish -
Immunogen
Synthetic peptide within Human Fbx32 aa 300 to the C-terminus (internal sequence) conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
(Peptide available asab74022) -
Positive control
- WB: Human, mouse and rat skeletal muscle tissue lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab74023 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (3) |
Use a concentration of 2 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).
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Notes |
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WB
Use a concentration of 2 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa). |
Target
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Function
Substrate recognition component of a (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Probably recognizes and binds to phosphorylated target proteins during skeletal muscle atrophy. Recognizes TERF1. -
Tissue specificity
Specifically expressed in cardiac and skeletal muscle. -
Pathway
Protein modification; protein ubiquitination. -
Sequence similarities
Contains 1 F-box domain. - Information by UniProt
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Database links
- Entrez Gene: 513776 Cow
- Entrez Gene: 114907 Human
- Entrez Gene: 67731 Mouse
- Entrez Gene: 733657 Pig
- Entrez Gene: 171043 Rat
- Omim: 606604 Human
- SwissProt: Q2KHT6 Cow
- SwissProt: Q969P5 Human
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Alternative names
- 4833442G10Rik antibody
- AI430017 antibody
- Atrogin 1 antibody
see all
Images
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All lanes : Anti-Fbx32 antibody (ab74023) at 2 µg/ml
Lane 1 : Human skeletal muscle tissue lysate - total protein (ab29330)
Lane 2 : Mouse skeletal muscle tissue lysate
Lane 3 : Rat skeletal muscle tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 42 kDa
Observed band size: 42 kDa
Additional bands at: 90 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 15 minutes
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (29)
ab74023 has been referenced in 29 publications.
- Testa MTJ et al. Resistance Training Attenuates Activation of STAT3 and Muscle Atrophy in Tumor-Bearing Mice. Front Oncol 12:880787 (2022). PubMed: 35847939
- Khin PP et al. Dulaglutide improves muscle function by attenuating inflammation through OPA-1-TLR-9 signaling in aged mice. Aging (Albany NY) 13:21962-21974 (2021). PubMed: 34537761
- Müller E et al. E3-ligase knock down revealed differential titin degradation by autopagy and the ubiquitin proteasome system. Sci Rep 11:21134 (2021). PubMed: 34702928
- Su J et al. The rat model of COPD skeletal muscle dysfunction induced by progressive cigarette smoke exposure: a pilot study. BMC Pulm Med 20:74 (2020). PubMed: 32293377
- Zhou L et al. Herbal complex 'Buyang Huanwu Tang' improves motor endplate function of denervated-dependent skeletal muscle atrophy in rat. J Integr Neurosci 19:89-99 (2020). PubMed: 32259889