RecombinantRabMAb

Recombinant Anti-Fbx32 antibody [EPR9148(2)] (ab168372)

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Western blot - Anti-Fbx32 antibody [EPR9148(2)] (ab168372)
  • Immunocytochemistry/ Immunofluorescence - Anti-Fbx32 antibody [EPR9148(2)] (ab168372)
  • Western blot - Anti-Fbx32 antibody [EPR9148(2)] (ab168372)
  • Immunocytochemistry/ Immunofluorescence - Anti-Fbx32 antibody [EPR9148(2)] (ab168372)
  • Western blot - Anti-Fbx32 antibody [EPR9148(2)] (ab168372)
  • Anti-Fbx32 antibody [EPR9148(2)] (ab168372)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9148(2)] to Fbx32
  • Suitable for: WB, ICC/IF
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free HRP

Overview

  • Product name

    Anti-Fbx32 antibody [EPR9148(2)]
    See all Fbx32 primary antibodies

  • Description

    Rabbit monoclonal [EPR9148(2)] to Fbx32

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IFmore details
    Unsuitable for: IHC-P

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide corresponding to Human Fbx32.
    Database link: Q969P5

  • Positive control

    • Human skeletal muscle, mouse heart, mouse muscle and rat muscle lysates; A673 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab168372 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (2)
1/1000 - 1/2000. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).
ICC/IF
1/200.
Notes
WB
1/1000 - 1/2000. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).
ICC/IF
1/200.
  • Application notes
    Is unsuitable for IHC-P.

    • Target

    • Function

      Substrate recognition component of a (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Probably recognizes and binds to phosphorylated target proteins during skeletal muscle atrophy. Recognizes TERF1.

    • Tissue specificity

      Specifically expressed in cardiac and skeletal muscle.

    • Pathway

      Protein modification; protein ubiquitination.

    • Sequence similarities

      Contains 1 F-box domain.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • 4833442G10Rik antibody
      • AI430017 antibody
      • Atrogin 1 antibody
      • Atrogin-1 antibody
      • ATROGIN1 antibody
      • Atrophy gene 1 antibody
      • F box only protein 32 antibody
      • F-box only protein 32 antibody
      • F-box protein 32 antibody
      • FBX32_HUMAN antibody
      • fbxo25 antibody
      • FBXO32 antibody
      • FLJ32424 antibody
      • MAFbx antibody
      • MGC108443 antibody
      • MGC137646 antibody
      • MGC33610 antibody
      • Muscle atrophy F box antibody
      • Muscle atrophy F box protein antibody
      • Muscle atrophy F-box protein antibody
      see all

    Images

    • Western blot - Anti-Fbx32 antibody [EPR9148(2)] (ab168372)
      Western blot - Anti-Fbx32 antibody [EPR9148(2)] (ab168372)
      All lanes : Anti-Fbx32 antibody [EPR9148(2)] (ab168372) at 1/1000 dilution (Purified)

      Lane 1 : Mouse muscle
      Lane 2 : Rat muscle

      Lysates/proteins at 20 µg per lane.

      Predicted band size: 42 kDa
      Observed band size: 42 kDa



      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • Immunocytochemistry/ Immunofluorescence - Anti-Fbx32 antibody [EPR9148(2)] (ab168372)
      Immunocytochemistry/ Immunofluorescence - Anti-Fbx32 antibody [EPR9148(2)] (ab168372)

      Immunofluorescent staining of A673 cells (fixed in 4% PFA) using purified ab168372 at a dilution of 1/200. An Alexa Fluor® 555 goat anti-rabbit (ab150082) was used at a dilution of 1/500 and the cells were counter-stained with DAPI. The negative control is shown in the bottom right hand panel - for the negative control, purified ab168372 was used at a dilution of 1/200 followed by an Alexa Fluor® 488 goat anti-mouse antibody at a dilution of 1/400.

    • Western blot - Anti-Fbx32 antibody [EPR9148(2)] (ab168372)
      Western blot - Anti-Fbx32 antibody [EPR9148(2)] (ab168372)
      Anti-Fbx32 antibody [EPR9148(2)] (ab168372) at 1/1000 dilution (Purified) + Human skeletal muscle at 10 µg

      Secondary
      HRP anti-rabbit IgG (specific to non-reduced IgG) at 1/1000 dilution

      Predicted band size: 42 kDa
      Observed band size: 42 kDa



      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • Immunocytochemistry/ Immunofluorescence - Anti-Fbx32 antibody [EPR9148(2)] (ab168372)
      Immunocytochemistry/ Immunofluorescence - Anti-Fbx32 antibody [EPR9148(2)] (ab168372)

      Immunofluorescent analysis of A673 cells labeling Fbx32 with ab168372 at 1/250 dilution.

    • Western blot - Anti-Fbx32 antibody [EPR9148(2)] (ab168372)
      Western blot - Anti-Fbx32 antibody [EPR9148(2)] (ab168372)
      All lanes : Anti-Fbx32 antibody [EPR9148(2)] (ab168372) at 1/1000 dilution (unpurified)

      Lane 1 : mouse heart lysate
      Lane 2 : rat muscle lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 42 kDa

    • Anti-Fbx32 antibody [EPR9148(2)] (ab168372)
      Anti-Fbx32 antibody [EPR9148(2)] (ab168372)

    References (43)

    Publishing research using ab168372? Please let us know so that we can cite the reference in this datasheet.

    ab168372 has been referenced in 43 publications.

    • Chen L  et al. Cryptotanshinone prevents muscle wasting in CT26-induced cancer cachexia through inhibiting STAT3 signaling pathway. J Ethnopharmacol 260:113066 (2020). PubMed: 32505837
    • Balboa E  et al. Vitamin E Blocks Connexin Hemichannels and Prevents Deleterious Effects of Glucocorticoid Treatment on Skeletal Muscles. Int J Mol Sci 21:N/A (2020). PubMed: 32521774
    • Wang M  et al. 20(S)-ginsenoside Rg3 promotes myoblast differentiation and protects against myotube atrophy via regulation of the Akt/mTOR/FoxO3 pathway. Biochem Pharmacol 180:114145 (2020). PubMed: 32653593
    • Shang GK  et al. Sarcopenia is attenuated by TRB3 knockout in aging mice via the alleviation of atrophy and fibrosis of skeletal muscles. J Cachexia Sarcopenia Muscle 11:1104-1120 (2020). PubMed: 32096609
    • Gonzalez-Ruiz C  et al. (-)-Epicatechin reduces muscle waste after complete spinal cord transection in a murine model: role of ubiquitin-proteasome system. Mol Biol Rep 47:8975-8985 (2020). PubMed: 33151476
    View all Publications for this product

    Customer reviews and Q&As

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    1-2 of 2 Abreviews or Q&A

    Western blot abreview for Anti-Fbx32 antibody [EPR9148(2)]

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rat Tissue lysate - other (muscle)
    Gel Running Conditions
    Reduced Denaturing (9)
    Loading amount
    30 µg
    Specification
    muscle
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Read More

    Mr. Baptiste Jude

    Verified customer

    Submitted Oct 10 2016

    Western blot abreview for Anti-Fbx32 [EPR9148(2)] antibody

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    20 µg
    Gel Running Conditions
    Reduced Denaturing (8)
    Sample
    Mouse Tissue lysate - whole (Heart)
    Specification
    Heart
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

    Abcam user community

    Verified customer

    Submitted Oct 14 2013

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