Recombinant Anti-Fbx32 antibody [EPR9148(2)] (ab168372)
![Western blot - Anti-Fbx32 antibody [EPR9148(2)] (ab168372)](https://www.abcam.com/ps/products/168/ab168372/Images/ab168372-237033-anti-fbx32-antibody-epr91482-western-blot.jpg "Western blot - Anti-Fbx32 antibody [EPR9148(2)] (ab168372)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9148(2)] to Fbx32
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Fbx32 antibody [EPR9148(2)]
See all Fbx32 primary antibodies -
Description
Rabbit monoclonal [EPR9148(2)] to Fbx32 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to Human Fbx32.
Database link: Q969P5 -
Positive control
- Human skeletal muscle, mouse heart, mouse muscle and rat muscle lysates; A673 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, 59% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR9148(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab168372 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (2) |
1/1000 - 1/2000. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).
|
| ICC/IF |
1/200.
|
| Notes |
|---|
|
WB
1/1000 - 1/2000. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa). |
|
ICC/IF
1/200. |
Target
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Function
Substrate recognition component of a (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Probably recognizes and binds to phosphorylated target proteins during skeletal muscle atrophy. Recognizes TERF1. -
Tissue specificity
Specifically expressed in cardiac and skeletal muscle. -
Pathway
Protein modification; protein ubiquitination. -
Sequence similarities
Contains 1 F-box domain. - Information by UniProt
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Database links
- Entrez Gene: 114907 Human
- Entrez Gene: 67731 Mouse
- Entrez Gene: 171043 Rat
- Omim: 606604 Human
- SwissProt: Q969P5 Human
- SwissProt: Q9CPU7 Mouse
- SwissProt: Q91Z62 Rat
- Unigene: 403933 Human
see all -
Alternative names
- 4833442G10Rik antibody
- AI430017 antibody
- Atrogin 1 antibody
see all
Images
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Western blot - Anti-Fbx32 antibody [EPR9148(2)] (ab168372)All lanes : Anti-Fbx32 antibody [EPR9148(2)] (ab168372) at 1/1000 dilution (Purified)
Lane 1 : Mouse muscle
Lane 2 : Rat muscle
Lysates/proteins at 20 µg per lane.
Predicted band size: 42 kDa
Observed band size: 42 kDaBlocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
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![Immunocytochemistry/ Immunofluorescence - Anti-Fbx32 antibody [EPR9148(2)] (ab168372)](https://www.abcam.com/ps/products/168/ab168372/Images/ab168372-237036-anti-fbx32-antibody-epr91482-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Fbx32 antibody [EPR9148(2)] (ab168372)Immunofluorescent staining of A673 cells (fixed in 4% PFA) using purified ab168372 at a dilution of 1/200. An Alexa Fluor® 555 goat anti-rabbit (ab150082) was used at a dilution of 1/500 and the cells were counter-stained with DAPI. The negative control is shown in the bottom right hand panel - for the negative control, purified ab168372 was used at a dilution of 1/200 followed by an Alexa Fluor® 488 goat anti-mouse antibody at a dilution of 1/400.
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![Western blot - Anti-Fbx32 antibody [EPR9148(2)] (ab168372)](https://www.abcam.com/ps/products/168/ab168372/Images/ab168372-237032-anti-fbx32-antibody-epr91482-western-blot.jpg)
Western blot - Anti-Fbx32 antibody [EPR9148(2)] (ab168372)Anti-Fbx32 antibody [EPR9148(2)] (ab168372) at 1/1000 dilution (Purified) + Human skeletal muscle at 10 µg
Secondary
HRP anti-rabbit IgG (specific to non-reduced IgG) at 1/1000 dilution
Predicted band size: 42 kDa
Observed band size: 42 kDaBlocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
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![Immunocytochemistry/ Immunofluorescence - Anti-Fbx32 antibody [EPR9148(2)] (ab168372)](https://www.abcam.com/ps/products/168/ab168372/Images/ab168372-169059-anti-fbx32-antibody-epr91482-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Fbx32 antibody [EPR9148(2)] (ab168372)Immunofluorescent analysis of A673 cells labeling Fbx32 with ab168372 at 1/250 dilution.
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![Western blot - Anti-Fbx32 antibody [EPR9148(2)] (ab168372)](https://www.abcam.com/ps/products/168/ab168372/Images/ab168372-169057-anti-fbx32-antibody-epr91482-western-blot.jpg)
Western blot - Anti-Fbx32 antibody [EPR9148(2)] (ab168372)All lanes : Anti-Fbx32 antibody [EPR9148(2)] (ab168372) at 1/1000 dilution (unpurified)
Lane 1 : mouse heart lysate
Lane 2 : rat muscle lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 42 kDa -
![Anti-Fbx32 antibody [EPR9148(2)] (ab168372)](https://www.abcam.com/ps/products/168/ab168372/Images/ab168372-8-benefits-of-recombinant-antibodies.png)
Anti-Fbx32 antibody [EPR9148(2)] (ab168372)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (58)
ab168372 has been referenced in 58 publications.
- Liu L et al. Formononetin ameliorates muscle atrophy by regulating myostatin-mediated PI3K/Akt/FoxO3a pathway and satellite cell function in chronic kidney disease. J Cell Mol Med 25:1493-1506 (2021). PubMed: 33405354
- Li Y et al. Role of miRNAs and lncRNAs in dexamethasone-induced myotube atrophy in vitro. Exp Ther Med 21:146 (2021). PubMed: 33456513
- Habel N et al. FBXO32 links ubiquitination to epigenetic reprograming of melanoma cells. Cell Death Differ 28:1837-1848 (2021). PubMed: 33462405
- Zeng X et al. Inhibition of mitochondrial and cytosolic calpain attenuates atrophy in myotubes co-cultured with colon carcinoma cells. Oncol Lett 21:124 (2021). PubMed: 33552245
- Wang M et al. 20(s)-ginseonside-Rg3 modulation of AMPK/FoxO3 signaling to attenuate mitochondrial dysfunction in a dexamethasone-injured C2C12 myotube-based model of skeletal atrophy in vitro. Mol Med Rep 23:N/A (2021). PubMed: 33649814