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  1. Link

    fbx32-antibody-epr91482-ab168372.pdf

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Cell Biology Proteolysis / Ubiquitin Proteasome / Ubiquitin Ubiquitin E3 Enzymes SCF Complex E3 Ligase
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RecombinantRabMAb

Recombinant Anti-Fbx32 antibody [EPR9148(2)] (ab168372)

  • Datasheet
  • SDS
Reviews (2) Submit a question References (58)

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Western blot - Anti-Fbx32 antibody [EPR9148(2)] (ab168372)
  • Immunocytochemistry/ Immunofluorescence - Anti-Fbx32 antibody [EPR9148(2)] (ab168372)
  • Western blot - Anti-Fbx32 antibody [EPR9148(2)] (ab168372)
  • Immunocytochemistry/ Immunofluorescence - Anti-Fbx32 antibody [EPR9148(2)] (ab168372)
  • Western blot - Anti-Fbx32 antibody [EPR9148(2)] (ab168372)
  • Anti-Fbx32 antibody [EPR9148(2)] (ab168372)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9148(2)] to Fbx32
  • Suitable for: WB, ICC/IF
  • Reacts with: Mouse, Rat, Human

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Overview

  • Product name

    Anti-Fbx32 antibody [EPR9148(2)]
    See all Fbx32 primary antibodies
  • Description

    Rabbit monoclonal [EPR9148(2)] to Fbx32
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
    Unsuitable for: IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human Fbx32.
    Database link: Q969P5

  • Positive control

    • Human skeletal muscle, mouse heart, mouse muscle and rat muscle lysates; A673 cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 0.05% BSA, 59% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR9148(2)
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteasome / Ubiquitin
    • Ubiquitin E3 Enzymes
    • SCF Complex E3 Ligase
    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteasome / Ubiquitin
    • Ubiquitin E3 Enzymes
    • F Box E3 Ligase
    • Stem Cells
    • Mesenchymal Stem Cells
    • Myogenesis
    • Cardiovascular
    • Heart
    • Hypertrophy
    • Other

Associated products

  • Alternative Versions

    • HRP Anti-Fbx32 antibody [EPR9148(2)] (ab198958)
    • Anti-Fbx32 antibody [EPR9148(2)] - BSA and Azide free (ab240956)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 555) preadsorbed (ab150082)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • Mouse heart normal tissue lysate - total protein (ab30291)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab168372 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (2)
1/1000 - 1/2000. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).
ICC/IF
1/200.
Notes
WB
1/1000 - 1/2000. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).
ICC/IF
1/200.
Application notes
Is unsuitable for IHC-P.

Target

  • Function

    Substrate recognition component of a (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Probably recognizes and binds to phosphorylated target proteins during skeletal muscle atrophy. Recognizes TERF1.
  • Tissue specificity

    Specifically expressed in cardiac and skeletal muscle.
  • Pathway

    Protein modification; protein ubiquitination.
  • Sequence similarities

    Contains 1 F-box domain.
  • Target information above from: UniProt accession Q969P5 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 114907 Human
    • Entrez Gene: 67731 Mouse
    • Entrez Gene: 171043 Rat
    • Omim: 606604 Human
    • SwissProt: Q969P5 Human
    • SwissProt: Q9CPU7 Mouse
    • SwissProt: Q91Z62 Rat
    • Unigene: 403933 Human
    • Unigene: 292042 Mouse
    • Unigene: 72619 Rat
    see all
  • Alternative names

    • 4833442G10Rik antibody
    • AI430017 antibody
    • Atrogin 1 antibody
    • Atrogin-1 antibody
    • ATROGIN1 antibody
    • Atrophy gene 1 antibody
    • F box only protein 32 antibody
    • F-box only protein 32 antibody
    • F-box protein 32 antibody
    • FBX32_HUMAN antibody
    • fbxo25 antibody
    • FBXO32 antibody
    • FLJ32424 antibody
    • MAFbx antibody
    • MGC108443 antibody
    • MGC137646 antibody
    • MGC33610 antibody
    • Muscle atrophy F box antibody
    • Muscle atrophy F box protein antibody
    • Muscle atrophy F-box protein antibody
    see all

Images

  • Western blot - Anti-Fbx32 antibody [EPR9148(2)] (ab168372)
    Western blot - Anti-Fbx32 antibody [EPR9148(2)] (ab168372)
    All lanes : Anti-Fbx32 antibody [EPR9148(2)] (ab168372) at 1/1000 dilution (Purified)

    Lane 1 : Mouse muscle
    Lane 2 : Rat muscle

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 42 kDa
    Observed band size: 42 kDa



    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

  • Immunocytochemistry/ Immunofluorescence - Anti-Fbx32 antibody [EPR9148(2)] (ab168372)
    Immunocytochemistry/ Immunofluorescence - Anti-Fbx32 antibody [EPR9148(2)] (ab168372)

    Immunofluorescent staining of A673 cells (fixed in 4% PFA) using purified ab168372 at a dilution of 1/200. An Alexa Fluor® 555 goat anti-rabbit (ab150082) was used at a dilution of 1/500 and the cells were counter-stained with DAPI. The negative control is shown in the bottom right hand panel - for the negative control, purified ab168372 was used at a dilution of 1/200 followed by an Alexa Fluor® 488 goat anti-mouse antibody at a dilution of 1/400.

  • Western blot - Anti-Fbx32 antibody [EPR9148(2)] (ab168372)
    Western blot - Anti-Fbx32 antibody [EPR9148(2)] (ab168372)
    Anti-Fbx32 antibody [EPR9148(2)] (ab168372) at 1/1000 dilution (Purified) + Human skeletal muscle at 10 µg

    Secondary
    HRP anti-rabbit IgG (specific to non-reduced IgG) at 1/1000 dilution

    Predicted band size: 42 kDa
    Observed band size: 42 kDa



    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

  • Immunocytochemistry/ Immunofluorescence - Anti-Fbx32 antibody [EPR9148(2)] (ab168372)
    Immunocytochemistry/ Immunofluorescence - Anti-Fbx32 antibody [EPR9148(2)] (ab168372)

    Immunofluorescent analysis of A673 cells labeling Fbx32 with ab168372 at 1/250 dilution.

  • Western blot - Anti-Fbx32 antibody [EPR9148(2)] (ab168372)
    Western blot - Anti-Fbx32 antibody [EPR9148(2)] (ab168372)
    All lanes : Anti-Fbx32 antibody [EPR9148(2)] (ab168372) at 1/1000 dilution (unpurified)

    Lane 1 : mouse heart lysate
    Lane 2 : rat muscle lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 42 kDa

  • Anti-Fbx32 antibody [EPR9148(2)] (ab168372)
    Anti-Fbx32 antibody [EPR9148(2)] (ab168372)

Protocols

  • Western blot protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (58)

Publishing research using ab168372? Please let us know so that we can cite the reference in this datasheet.

ab168372 has been referenced in 58 publications.

  • Liu L  et al. Formononetin ameliorates muscle atrophy by regulating myostatin-mediated PI3K/Akt/FoxO3a pathway and satellite cell function in chronic kidney disease. J Cell Mol Med 25:1493-1506 (2021). PubMed: 33405354
  • Li Y  et al. Role of miRNAs and lncRNAs in dexamethasone-induced myotube atrophy in vitro. Exp Ther Med 21:146 (2021). PubMed: 33456513
  • Habel N  et al. FBXO32 links ubiquitination to epigenetic reprograming of melanoma cells. Cell Death Differ 28:1837-1848 (2021). PubMed: 33462405
  • Zeng X  et al. Inhibition of mitochondrial and cytosolic calpain attenuates atrophy in myotubes co-cultured with colon carcinoma cells. Oncol Lett 21:124 (2021). PubMed: 33552245
  • Wang M  et al. 20(s)-ginseonside-Rg3 modulation of AMPK/FoxO3 signaling to attenuate mitochondrial dysfunction in a dexamethasone-injured C2C12 myotube-based model of skeletal atrophy in vitro. Mol Med Rep 23:N/A (2021). PubMed: 33649814
View all Publications for this product

Customer reviews and Q&As

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1-2 of 2 Abreviews or Q&A

Western blot abreview for Anti-Fbx32 antibody [EPR9148(2)]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Rat Tissue lysate - other (muscle)
Gel Running Conditions
Reduced Denaturing (9)
Loading amount
30 µg
Specification
muscle
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Read More

MR. Baptiste Jude

Verified customer

Submitted Oct 10 2016

Western blot abreview for Anti-Fbx32 [EPR9148(2)] antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (8)
Sample
Mouse Tissue lysate - whole (Heart)
Specification
Heart
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Read More

Abcam user community

Verified customer

Submitted Oct 14 2013

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