RecombinantRabMAb

Recombinant Anti-Fbx32 antibody [EPR9148(2)] - BSA and Azide free (ab240956)

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Immunocytochemistry/ Immunofluorescence - Anti-Fbx32 antibody [EPR9148(2)] - BSA and Azide free (ab240956)
  • Immunocytochemistry/ Immunofluorescence - Anti-Fbx32 antibody [EPR9148(2)] - BSA and Azide free (ab240956)
  • Anti-Fbx32 antibody [EPR9148(2)] - BSA and Azide free (ab240956)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9148(2)] to Fbx32 - BSA and Azide free
  • Suitable for: WB, ICC/IF
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Fbx32 antibody [EPR9148(2)] - BSA and Azide free
    See all Fbx32 primary antibodies

  • Description

    Rabbit monoclonal [EPR9148(2)] to Fbx32 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IFmore details
    Unsuitable for: IHC-P

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human Fbx32. The exact sequence is proprietary.
    Database link: Q969P5

  • Positive control

    • Human skeletal muscle, mouse heart, mouse muscle and rat muscle lysates; A673 cells.

  • General notes

    Ab240956 is the carrier-free version of ab168372. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab240956 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab240956 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).
ICC/IF Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for IHC-P.

    • Target

    • Function

      Substrate recognition component of a (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Probably recognizes and binds to phosphorylated target proteins during skeletal muscle atrophy. Recognizes TERF1.

    • Tissue specificity

      Specifically expressed in cardiac and skeletal muscle.

    • Pathway

      Protein modification; protein ubiquitination.

    • Sequence similarities

      Contains 1 F-box domain.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • 4833442G10Rik antibody
      • AI430017 antibody
      • Atrogin 1 antibody
      • Atrogin-1 antibody
      • ATROGIN1 antibody
      • Atrophy gene 1 antibody
      • F box only protein 32 antibody
      • F-box only protein 32 antibody
      • F-box protein 32 antibody
      • FBX32_HUMAN antibody
      • fbxo25 antibody
      • FBXO32 antibody
      • FLJ32424 antibody
      • MAFbx antibody
      • MGC108443 antibody
      • MGC137646 antibody
      • MGC33610 antibody
      • Muscle atrophy F box antibody
      • Muscle atrophy F box protein antibody
      • Muscle atrophy F-box protein antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-Fbx32 antibody [EPR9148(2)] - BSA and Azide free (ab240956)
      Immunocytochemistry/ Immunofluorescence - Anti-Fbx32 antibody [EPR9148(2)] - BSA and Azide free (ab240956)

      Immunofluorescent analysis of A673 cells labeling Fbx32 with ab168372 at 1/250 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab168372)
    • Immunocytochemistry/ Immunofluorescence - Anti-Fbx32 antibody [EPR9148(2)] - BSA and Azide free (ab240956)
      Immunocytochemistry/ Immunofluorescence - Anti-Fbx32 antibody [EPR9148(2)] - BSA and Azide free (ab240956)

      Immunofluorescent staining of A673 cells (fixed in 4% PFA) using purified ab168372 at a dilution of 1/200. An Alexa Fluor® 555 goat anti-rabbit (ab150082) was used at a dilution of 1/500 and the cells were counter-stained with DAPI. The negative control is shown in the bottom right hand panel - for the negative control, purified ab168372 was used at a dilution of 1/200 followed by an Alexa Fluor® 488 goat anti-mouse antibody at a dilution of 1/400.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab168372)
    • Anti-Fbx32 antibody [EPR9148(2)] - BSA and Azide free (ab240956)
      Anti-Fbx32 antibody [EPR9148(2)] - BSA and Azide free (ab240956)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab240956? Please let us know so that we can cite the reference in this datasheet.

    ab240956 has not yet been referenced specifically in any publications.

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