Recombinant Anti-Fbx32 antibody [EPR9148(2)] - BSA and Azide free (ab240956)
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Overview
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Product name
Anti-Fbx32 antibody [EPR9148(2)] - BSA and Azide free
See all Fbx32 primary antibodies -
Description
Rabbit monoclonal [EPR9148(2)] to Fbx32 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human Fbx32. The exact sequence is proprietary.
Database link: Q969P5 -
Positive control
- Human skeletal muscle, mouse heart, mouse muscle and rat muscle lysates; A673 cells.
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General notes
Ab240956 is the carrier-free version of ab168372. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab240956 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR9148(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab240956 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use at an assay dependent concentration. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa). | |
| ICC/IF | Use at an assay dependent concentration. |
Target
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Function
Substrate recognition component of a (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Probably recognizes and binds to phosphorylated target proteins during skeletal muscle atrophy. Recognizes TERF1. -
Tissue specificity
Specifically expressed in cardiac and skeletal muscle. -
Pathway
Protein modification; protein ubiquitination. -
Sequence similarities
Contains 1 F-box domain. - Information by UniProt
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Database links
- Entrez Gene: 114907 Human
- Entrez Gene: 67731 Mouse
- Entrez Gene: 171043 Rat
- Omim: 606604 Human
- SwissProt: Q969P5 Human
- SwissProt: Q9CPU7 Mouse
- SwissProt: Q91Z62 Rat
- Unigene: 403933 Human
see all -
Alternative names
- 4833442G10Rik antibody
- AI430017 antibody
- Atrogin 1 antibody
see all
Images
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![Immunocytochemistry/ Immunofluorescence - Anti-Fbx32 antibody [EPR9148(2)] - BSA and Azide free (ab240956)](https://www.abcam.com/ps/products/240/ab240956/Images/ab240956-2-ab168372169059ab168372e.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Fbx32 antibody [EPR9148(2)] - BSA and Azide free (ab240956) -
![Immunocytochemistry/ Immunofluorescence - Anti-Fbx32 antibody [EPR9148(2)] - BSA and Azide free (ab240956)](https://www.abcam.com/ps/products/240/ab240956/Images/ab240956-1-ab168372237036168372ICC1.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Fbx32 antibody [EPR9148(2)] - BSA and Azide free (ab240956)Immunofluorescent staining of A673 cells (fixed in 4% PFA) using purified ab168372 at a dilution of 1/200. An Alexa Fluor® 555 goat anti-rabbit (ab150082) was used at a dilution of 1/500 and the cells were counter-stained with DAPI. The negative control is shown in the bottom right hand panel - for the negative control, purified ab168372 was used at a dilution of 1/200 followed by an Alexa Fluor® 488 goat anti-mouse antibody at a dilution of 1/400.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab168372)
Datasheets and documents
References
ab240956 has not yet been referenced specifically in any publications.
