Overview

  • Product name

    Anti-Fbx32 antibody [EPR9148(2)] (HRP)
    See all Fbx32 primary antibodies
  • Description

    Rabbit monoclonal [EPR9148(2)] to Fbx32 (HRP)
  • Host species

    Rabbit
  • Conjugation

    HRP
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human Fbx32.
    Database link: Q969P5

  • Positive control

    • WB: Human Skeletal Muscle, Mouse Skeletal Muscle, Rat Skeletal Muscle, Human Heart, Mouse Heart and Rat Heart tissue lysates. IHC-P: FFPE human heart muscle (normal) and human skeletal muscle (normal) tissue sections.
  • General notes

     

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab198958 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).
IHC-P 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Substrate recognition component of a (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Probably recognizes and binds to phosphorylated target proteins during skeletal muscle atrophy. Recognizes TERF1.
  • Tissue specificity

    Specifically expressed in cardiac and skeletal muscle.
  • Pathway

    Protein modification; protein ubiquitination.
  • Sequence similarities

    Contains 1 F-box domain.
  • Information by UniProt
  • Database links

  • Alternative names

    • 4833442G10Rik antibody
    • AI430017 antibody
    • Atrogin 1 antibody
    • Atrogin-1 antibody
    • ATROGIN1 antibody
    • Atrophy gene 1 antibody
    • F box only protein 32 antibody
    • F-box only protein 32 antibody
    • F-box protein 32 antibody
    • FBX32_HUMAN antibody
    • fbxo25 antibody
    • FBXO32 antibody
    • FLJ32424 antibody
    • MAFbx antibody
    • MGC108443 antibody
    • MGC137646 antibody
    • MGC33610 antibody
    • Muscle atrophy F box antibody
    • Muscle atrophy F box protein antibody
    • Muscle atrophy F-box protein antibody
    see all

Images

  • IHC image of Fbx32 staining in a section of formalin-fixed paraffin-embedded normal human skeletal muscle*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins, and incubated overnight at +4°C with ab198958 at 1/100 dilution. DAB was used as the chromogen (ab103723), diluted 1/100 and incubated for 10min at room temperature. The section was counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • IHC image of Fbx32 staining in a section of formalin-fixed paraffin-embedded normal human heart muscle. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins, and incubated overnight at +4°C with ab198958 at 1/100 dilution. DAB was used as the chromogen (ab103723), diluted 1/100 and incubated for 10min at room temperature. The section was counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • All lanes : Anti-Fbx32 antibody [EPR9148(2)] (HRP) (ab198958) at 1/5000 dilution

    Lane 1 : Human skeletal muscle tissue lysate - total protein (ab29330)
    Lane 2 : Skeletal Muscle (Mouse) Tissue Lysate
    Lane 3 : Skeletal Muscle (Rat) Tissue Lysate
    Lane 4 : Human heart tissue lysate - total protein (ab29431)
    Lane 5 : Heart (Mouse) Tissue Lysate
    Lane 6 : Heart (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 42 kDa
    Observed band size: 42 kDa


    Exposure time: 30 seconds


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab198958 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

References

This product has been referenced in:

  • Kim HE  et al. Upregulation of Atrogin-1/FBXO32 is not necessary for cartilage destruction in mouse models of osteoarthritis. Osteoarthritis Cartilage 25:397-400 (2017). Read more (PubMed: 27480933) »
See 1 Publication for this product

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