Overview

  • Product name
  • Description
    Goat polyclonal to FBXL11
  • Host species
    Goat
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Hamster, Dog, Pig, Monkey
  • Immunogen

    Synthetic peptide corresponding to Human FBXL11 aa 445-459 (internal sequence) (Cysteine residue).
    Sequence:

    PRKDRQVHLTHFELE


    Database link: NP_036440.1

  • Positive control
    • Human small intestine tissue; Human uterus lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab174693 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 140 kDa (predicted molecular weight: 133 kDa).
IHC-P Use a concentration of 5 µg/ml.

Target

  • Function
    Histone demethylase that specifically demethylates 'Lys-36' of histone H3, thereby playing a central role in histone code. Preferentially demethylates dimethylated H3 'Lys-36' residue while it has weak or no activity for mono- and tri-methylated H3 'Lys-36'. May also recognize and bind to some phosphorylated proteins and promote their ubiquitination and degradation. Required to maintain the heterochromatic state. Associates with centromeres and represses transcription of small non-coding RNAs that are encoded by the clusters of satellite repeats at the centromere. Required to sustain centromeric integrity and genomic stability, particularly during mitosis.
  • Tissue specificity
    Widely expressed, with highest levels in brain, testis and ovary, followed by lung.
  • Sequence similarities
    Belongs to the JHDM1 histone demethylase family.
    Contains 1 CXXC-type zinc finger.
    Contains 1 F-box domain.
    Contains 1 JmjC domain.
    Contains 6 LRR (leucine-rich) repeats.
    Contains 1 PHD-type zinc finger.
  • Domain
    The JmjC domain mediates demethylation activity and is required for satellite silencing.
    The CXXC zinc finger preferentially recognizes nonmethylated CpG DNA, and binding is blocked when the CpG DNA is methylated.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Nucleus > nucleoplasm. Punctate expression throughout the nucleoplasm and enriched in the perinucleolar region. Specifically nucleates at CpG islands where it's presence results in chromatin depleted in H3K36me2.
  • Information by UniProt
  • Database links
  • Alternative names
    • [Histone-H3]-lysine-36 demethylase 1A antibody
    • CXXC-type zinc finger protein 8 antibody
    • CXXC8 antibody
    • F box / LRR repeat protein 11 antibody
    • F box and leucine rich repeat protein 11 antibody
    • F box protein FBL7 antibody
    • F-box and leucine-rich repeat protein 11 antibody
    • F-box protein FBL7 antibody
    • F-box protein Lilina antibody
    • F-box/LRR-repeat protein 11 antibody
    • FBL11 antibody
    • FBL7 antibody
    • FBXL11 antibody
    • JHDM1A antibody
    • JmjC domain-containing histone demethylation protein 1A antibody
    • Jumonji C domain containing histone demethylase 1A antibody
    • kdm2a antibody
    • KDM2A_HUMAN antibody
    • LILINA antibody
    • Lysine (K) specific demethylase 2A antibody
    • Lysine-specific demethylase 2A antibody
    see all

Images

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human small intestine tissue labeling FBXL11 with ab174693 at 5 µg/ml.

  • Anti-FBXL11 antibody (ab174693) at 0.5 µg/ml + Human uterus lysate (in RIPA buffer) at 35 µg

    Developed using the ECL technique.

    Predicted band size: 133 kDa
    Observed band size: 140 kDa (why is the actual band size different from the predicted?)



    Primary incubation was 1 hour.

References

ab174693 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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