Key features and details
- Rabbit polyclonal to FbxL12
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-FbxL12 antibody
DescriptionRabbit polyclonal to FbxL12
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat
- HepG2 and NIH-3T3 whole cell lysates; SW480; 293T, A431, H1299, HeLa, Molt-4 and Raji cell lysates
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 89.99% PBS, 10% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab96831 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/500.|
|WB||1/500 - 1/3000. Predicted molecular weight: 37 kDa.|
FunctionSubstrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex.
Sequence similaritiesContains 1 F-box domain.
Contains 8 LRR (leucine-rich) repeats.
- Information by UniProt
- F box and leucine rich repeat protein 12 antibody
- F box protein FBL12 antibody
- F box/LRR repeat protein 12 antibody
Anti-FbxL12 antibody (ab96831) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg
Predicted band size: 37 kDa
Anti-FbxL12 antibody (ab96831) at 1/1000 dilution + NIH-3T3 whole cell lysate at 30 µg
Predicted band size: 37 kDa
ab96831, at 1/500 dilution, staining FbxL12 in paraffin-embedded SW480 by Immunohistochemistry.
ab96831 has been referenced in 2 publications.
- Zhao B et al. Notch and the pre-TCR coordinate thymocyte proliferation by induction of the SCF subunits Fbxl1 and Fbxl12. Nat Immunol 20:1381-1392 (2019). PubMed: 31451788
- Tsuruta F et al. SCFFbl12 Increases p21Waf1/Cip1 Expression Level through Atypical Ubiquitin Chain Synthesis. Mol Cell Biol 36:2182-94 (2016). WB ; Human . PubMed: 27215384