Product nameAnti-FBXL16 antibody
See all FBXL16 primary antibodies
DescriptionRabbit polyclonal to FBXL16
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Rat, Human
Recombinant fragment within Human FBXL16 (internal sequence). The exact sequence is proprietary.
Database link: Q8N461
- ICC/IF: MCF7 cells. IHC-P: Rat brain tissue. WB: U-87 MG, SK-N-SH, IMR32, SK-N-AS cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: PBS, 1% BSA, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab272898 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000.|
|IHC-P||1/100 - 1/1000.|
|ICC/IF||1/100 - 1/1000.|
FunctionSubstrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex.
Sequence similaritiesContains 1 F-box domain.
Contains 7 LRR (leucine-rich) repeats.
- Information by UniProt
- BC042620 antibody
- C16orf22 antibody
- c380A1.1 (novel protein) antibody
Paraformaldehyde-fixed MCF7 cells stained for FBXL16 (green) using ab272898 at 1/500 dilution in ICC/IF analysis.
Paraffin embedded rat brain tissue stained for FBXL16 using ab272898 at 1/500 dilution in immunohistochemical analysis. Antigen retrieval using EDTA based, pH 8.0 buffer, 15 minutes.
All lanes : Anti-FBXL16 antibody (ab272898) at 1/1000 dilution
Lane 1 : U-87 MG cell lysate
Lane 2 : SK-N-SH cell lysate
Lane 3 : IMR32 cell lysate
Lane 4 : SK-N-AS cell lysate
Lysates/proteins at 30 µg per lane.
All lanes : HRP-conjugated anti-rabbit IgG antibody
Anti-FBXL16 antibody (ab272898) at 1/1000 dilution + U-87 MG cell lysate at 30 µg
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab272898 has not yet been referenced specifically in any publications.