Anti-FBXL17 antibody (ab111683)
Key features and details
- Rabbit polyclonal to FBXL17
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-FBXL17 antibody -
Description
Rabbit polyclonal to FBXL17 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Recombinant fragment, corresponding to a region within amino acids 1-156 of Human FBXL17, isoform 2 (AAI26145).
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Positive control
- MCF7 whole cell lysate; U373 xenograft tissue.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab111683 is purified by antigen-affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab111683 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | 1/500 - 1/3000. Predicted molecular weight: 34 kDa. | |
IHC-P | 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex. -
Sequence similarities
Contains 1 F-box domain. - Information by UniProt
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Database links
- Entrez Gene: 64839 Human
- Entrez Gene: 50758 Mouse
- Entrez Gene: 316663 Rat
- Omim: 609083 Human
- SwissProt: Q9UF56 Human
- SwissProt: Q9QZN1 Mouse
- Unigene: 657225 Human
- Unigene: 721914 Human
see all -
Alternative names
- DKFZP434C1715 antibody
- F box and leucine rich repeat protein 17 antibody
- F box only protein 13 antibody
see all
Images
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Anti-FBXL17 antibody (ab111683) at 1/1000 dilution + MCF7 whole cell lysate at 30 µg
Predicted band size: 34 kDa
10% SDS-PAGE. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FBXL17 antibody (ab111683)ab111683 at 1/500 dilution staining FBXL17 in paraffin-embedded U373 xenograft tissue by Immunohistochemistry.
Protocols
References (1)
ab111683 has been referenced in 1 publication.
- Lai Y et al. Lipopolysaccharide modulates p300 and Sirt1 to promote PRMT1 stability via an SCFFbxl17-recognized acetyldegron. J Cell Sci 130:3578-3587 (2017). PubMed: 28883095