Overview

  • Product name
    Anti-FBXL17 antibody
  • Description
    Rabbit polyclonal to FBXL17
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 1-156 of Human FBXL17, isoform 2 (AAI26145).

  • Positive control
    • MCF7 whole cell lysate; U373 xenograft tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 20% Glycerol, 0.75% Glycine
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab111683 is purified by antigen-affinity chromatography.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab111683 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 34 kDa.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

Images

  • Western blot - Anti-FBXL17 antibody (ab111683)
    Western blot - Anti-FBXL17 antibody (ab111683)
    Anti-FBXL17 antibody (ab111683) at 1/1000 dilution + MCF7 whole cell lysate at 30 µg

    Predicted band size: 34 kDa



    10% SDS-PAGE.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FBXL17 antibody (ab111683)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FBXL17 antibody (ab111683)
    ab111683 at 1/500 dilution staining FBXL17 in paraffin-embedded U373 xenograft tissue by Immunohistochemistry.

References

ab111683 has not yet been referenced specifically in any publications.

Publishing research using ab111683? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

Submit a review Submit a question

1 Review or Q&A

10
Votes: Highest First

WB with Hela cells sees multiple bands but none at 72 kDa, a weak at around 35 kDa (isoforms) used also siRNA (4 different sequences) to reduce signal, but saw no reduction at all, sees however biological phenotype with siRNA (but not in WB) Ab: 1/1...

Read More

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this h...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up