Overview

  • Product name

  • Description

    Goat polyclonal to FBXL2
  • Host species

    Goat
  • Tested applications

    Suitable for: IHC-P, ELISA, WBmore details
  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Cow, Dog
  • Immunogen

    Synthetic peptide: VFSNNDEGLINKK, corresponding to N terminal amino acids 2-14 of Human FBXL2.

  • Positive control

    • Human Kidney lysates

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline. pH 7.3
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab17018 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 - 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ELISA 1/32000.
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 50-55 kDa (predicted molecular weight: 49 kDa).

Target

  • Function

    Calcium-activated substrate recognition component of a SCF (SKP1-cullin-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Unlike many F-box proteins, FBXL2 doesn't seem to target phosphodegron within its substrates but rather calmodulin-binding motifs. Targets PCYT1A for its monoubiquitination and degradation, this is antagonized by calmodulin (By similarity). Targets the cyclins CCND2 and CCND3 for polyubiquitination and degradation, leading to cell-cycle arrest in G(0), also antagonized by calmodulin. Binds to hepatitis C virus non-structural protein 5A (NS5A) in a reaction crucial for hepatitis C virus RNA replication.
  • Tissue specificity

    Expressed in brain, heart, kidney, liver, lung, pancreas and placenta.
  • Sequence similarities

    Contains 1 F-box domain.
    Contains 13 LRR (leucine-rich) repeats.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • DKFZP564P0622 antibody
    • F box and leucine rich repeat protein 2 antibody
    • F box protein containing leucine rich repeats antibody
    • F box protein FBL2/FBL3 antibody
    • F box/LRR repeat protein 2 antibody
    • F-box and leucine-rich repeat protein 2 antibody
    • F-box protein FBL2/FBL3 antibody
    • F-box/LRR-repeat protein 2 antibody
    • FBL 2 antibody
    • FBL 3 antibody
    • FBL2 antibody
    • FBL3 antibody
    • FBXL 2 antibody
    • FBXL2 antibody
    • FBXL2_HUMAN antibody
    see all

Images

  • All lanes : Anti-FBXL2 antibody (ab17018) at 0.3 µg/ml

    Lane 1 : Human brain tissue lysate
    Lane 2 : Rat brain tissue lysate

    Lysates/proteins at 35 µg per lane.

    Predicted band size: 49 kDa



    Primary incubation was 1 hour. Detected by chemiluminescence.

  • ab17018 (10µg/ml) staining of FBXL2 in paraffin embedded Human Cerebral Cortex following steamed antigen retrieval with citrate buffer pH 6 and AP-staining shows cytoplasm staining in neuronal bodies.

  • ab17018 at 0.5µg/ml detecting FBXL2 from Human kidney lysate (35µg total protein per lane). Primary antibody was incubated for 1 hour and the blot was detected using chemiluminescence. ab17018 at 0.5µg/ml detecting FBXL2 from Human kidney lysate (35µg total protein per lane). Primary antibody was incubated for 1 hour and the blot was detected using chemiluminescence.

References

This product has been referenced in:

See 1 Publication for this product

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