Key features and details
- Goat polyclonal to FBXL2
- Suitable for: IHC-P, WB
- Reacts with: Rat, Human
- Isotype: IgG
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab17018 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 - 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 50-55 kDa (predicted molecular weight: 49 kDa).
1 hour primary incubation is recommended for this product.
FunctionCalcium-activated substrate recognition component of a SCF (SKP1-cullin-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Unlike many F-box proteins, FBXL2 doesn't seem to target phosphodegron within its substrates but rather calmodulin-binding motifs. Targets PCYT1A for its monoubiquitination and degradation, this is antagonized by calmodulin (By similarity). Targets the cyclins CCND2 and CCND3 for polyubiquitination and degradation, leading to cell-cycle arrest in G(0), also antagonized by calmodulin. Binds to hepatitis C virus non-structural protein 5A (NS5A) in a reaction crucial for hepatitis C virus RNA replication.
Tissue specificityExpressed in brain, heart, kidney, liver, lung, pancreas and placenta.
Sequence similaritiesContains 1 F-box domain.
Contains 13 LRR (leucine-rich) repeats.
- Information by UniProt
- DKFZP564P0622 antibody
- F box and leucine rich repeat protein 2 antibody
- F box protein containing leucine rich repeats antibody
All lanes : Anti-FBXL2 antibody (ab17018) at 0.3 µg/ml
Lane 1 : Human brain tissue lysate
Lane 2 : Rat brain tissue lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 49 kDa
Primary incubation was 1 hour. Detected by chemiluminescence.
ab17018 (10µg/ml) staining of FBXL2 in paraffin embedded Human Cerebral Cortex following steamed antigen retrieval with citrate buffer pH 6 and AP-staining shows cytoplasm staining in neuronal bodies.
ab17018 at 0.5
µg/ml detecting FBXL2 from Human kidney lysate (35 µg total protein per lane). Primary antibody was incubated for 1 hour and the blot was detected using chemiluminescence. ab17018 at 0.5µg/ml detecting FBXL2 from Human kidney lysate (35µg total protein per lane). Primary antibody was incubated for 1 hour and the blot was detected using chemiluminescence.
ab17018 has been referenced in 1 publication.
- Yu Q et al. miRNA-346 promotes proliferation, migration and invasion in liver cancer. Oncol Lett 14:3255-3260 (2017). PubMed: 28927074