Overview

  • Product name

    Anti-FBXL2 antibody - N-terminal
    See all FBXL2 primary antibodies
  • Description

    Rabbit polyclonal to FBXL2 - N-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Cow
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 1-44 of Human FBXL2.

  • Positive control

    • A431, HeLa, HepG2 and Raji cell lysates; HeLa cells; SW480 xenograft.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: PBS, 10% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab153842 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 47 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Calcium-activated substrate recognition component of a SCF (SKP1-cullin-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Unlike many F-box proteins, FBXL2 doesn't seem to target phosphodegron within its substrates but rather calmodulin-binding motifs. Targets PCYT1A for its monoubiquitination and degradation, this is antagonized by calmodulin (By similarity). Targets the cyclins CCND2 and CCND3 for polyubiquitination and degradation, leading to cell-cycle arrest in G(0), also antagonized by calmodulin. Binds to hepatitis C virus non-structural protein 5A (NS5A) in a reaction crucial for hepatitis C virus RNA replication.
  • Tissue specificity

    Expressed in brain, heart, kidney, liver, lung, pancreas and placenta.
  • Sequence similarities

    Contains 1 F-box domain.
    Contains 13 LRR (leucine-rich) repeats.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • DKFZP564P0622 antibody
    • F box and leucine rich repeat protein 2 antibody
    • F box protein containing leucine rich repeats antibody
    • F box protein FBL2/FBL3 antibody
    • F box/LRR repeat protein 2 antibody
    • F-box and leucine-rich repeat protein 2 antibody
    • F-box protein FBL2/FBL3 antibody
    • F-box/LRR-repeat protein 2 antibody
    • FBL 2 antibody
    • FBL 3 antibody
    • FBL2 antibody
    • FBL3 antibody
    • FBXL 2 antibody
    • FBXL2 antibody
    • FBXL2_HUMAN antibody
    see all

Images

  • All lanes : Anti-FBXL2 antibody - N-terminal (ab153842) at 1/2000 dilution

    Lane 1 : Hela whole cell lysate
    Lane 2 : Raji whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 47 kDa
    Observed band size: 47 kDa



    10% SDS PAGE
  • Immunofluorescent analysis of methanol-fixed HeLa cells labeling FBXL2 with ab153842 at 1/200 dilution. Lower panel is co-stained with Hoechst 33342.
  • Immunohistochemical analysis of paraffin-embedded SW480 xenograft labeling FBXL2 with ab153842 at 1/500 dilution.

References

ab153842 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab153842.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up