Product nameAnti-FBXL20 antibody
See all FBXL20 primary antibodies
DescriptionRabbit polyclonal to FBXL20
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
An 18 amino acid peptide near the carboxy terminus of human FBXL20.
- A20 cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferPreservative: 0.02% Sodium azide
Concentration information loading...
PurityImmunogen affinity purified
- Cell Biology
- Proteolysis / Ubiquitin
- Proteasome / Ubiquitin
- Ubiquitin E3 Enzymes
- SCF Complex E3 Ligase
Our Abpromise guarantee covers the use of ab67232 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 44, 48 kDa (predicted molecular weight: 44 , 48 kDa).|
|IHC-P||Use a concentration of 2.5 µg/ml.|
RelevanceFBXL20 is a member of the F-box protein family, which are characterized by an approximately 40-amino acid F-box motif. SCF complexes, formed by SKP1, cullin, and F-box proteins, act as protein-ubiquitin ligases. FBXL20 is selectively expressed in the brain, broadly expressed within the mouse CNS and is abundant at presynaptic membrane. FBXL20 has orthologs in C. elegans, D. melanogaster, and mammals which suggests that it might function as an important membrane-localized E3 ligase in various species. FBXL20 is a major presynaptic E3 ubiquitin ligase that acts through RIM1a via degradation and the ubiquitin-proteasome-system (UPS) pathway to critically regulate synaptic transmission. This identifies protein degradation as a mechanism for holding synaptic communication in check.
- F box and leucine rich repeat protein 20 antibody
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Lane 1 : Anti-FBXL20 antibody (ab67232) at 0.5 µg/ml
Lane 2 : Anti-FBXL20 antibody (ab67232) at 1 µg/ml
All lanes : A20 cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 44 , 48 kDa
Observed band size: 44 and 48 kDa why is the actual band size different from the predicted?
ab67232 at 2.5 µg/ml staining FBXL20 in human brain tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections).
ab67232 has not yet been referenced specifically in any publications.