Anti-FBXL22 antibody (ab223059)
Key features and details
- Rabbit polyclonal to FBXL22
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-FBXL22 antibody -
Description
Rabbit polyclonal to FBXL22 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment corresponding to Human FBXL22 aa 100 to the C-terminus.
Database link: Q6P050 -
Positive control
- WB: Mouse brain lysate; human placenta lysate. IHC-P: Human pancreatic cancer tissue. ICC/IF: HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purity>95% -
Clonality
Polyclonal -
Isotype
IgG
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab223059 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/5000. Predicted molecular weight: 27 kDa.
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IHC-P |
1/20 - 1/200.
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ICC/IF |
1/50 - 1/200.
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Notes |
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WB
1/1000 - 1/5000. Predicted molecular weight: 27 kDa. |
IHC-P
1/20 - 1/200. |
ICC/IF
1/50 - 1/200. |
Target
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Relevance
This gene encodes a member of the F-box protein family. This F-box protein interacts with S-phase kinase-associated protein 1A and cullin in order to form SCF complexes which function as ubiquitin ligases. -
Database links
- Entrez Gene: 283807 Human
- Entrez Gene: 74165 Mouse
- Omim: 609088 Human
- SwissProt: Q6P050 Human
- SwissProt: Q8C7B6 Mouse
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Alternative names
- F box and leucine rich repeat protein 22 antibody
- F box/LRR repeat protein 22 antibody
- Fbl22 antibody
- FLJ39626 antibody
Images
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All lanes : Anti-FBXL22 antibody (ab223059) at 1/1000 dilution
Lane 1 : Mouse brain lysate
Lane 2 : Human placenta lysate
Secondary
All lanes : Goat Anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 27 kDa -
Paraffin embedded human pancreatic cancer tissue stained for FBXL22 with ab223059 at 1/100 dilution in immunohistochemical analysis.
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HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for FBXL22 (green) using ab223059 at 1/100 dilution in ICC/IF. Secondary: Alexa Fluor® 488-conjugated Goat Anti-Rabbit IgG (H+L).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab223059 has not yet been referenced specifically in any publications.