Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: 1% BSA, PBS
Concentration information loading...
PurityProtein G purified
Purification notesPurified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG depleted (approximately 95%) fetal bovine serum
Our Abpromise guarantee covers the use of ab53541 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent dilution. Predicted molecular weight: 70 kDa.|
|Dot blot||Use at an assay dependent dilution.|
Tissue specificityExpressed in heart, kidney, liver, lung, pancreas, and placenta, but not in skeletal muscle.
Involvement in diseaseMitochondrial DNA depletion syndrome 13
Sequence similaritiesContains 1 F-box domain.
Contains 9 LRR (leucine-rich) repeats.
Cellular localizationCytoplasm. Nucleus. Mitochondrion.
- Information by UniProt
- F box and leucine rich repeat protein 4 antibody
- F box protein FBL4/FBL5 antibody
- F box/LRR repeat protein 4 antibody
Anti-FbxL4 antibody [2352C1a] (ab53541) + Immunized recombinant protein
Predicted band size: 70 kDa
Observed band size: 31 kDa why is the actual band size different from the predicted?
The molecular weight of the band on the Western blot does not correspond to the molecular weight of the natural protein as the immunizing recombinant protein fragment was used as the test antigen
ab53541 has not yet been referenced specifically in any publications.