Anti-FBXL5 antibody (ab140175)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FBXL5 antibody (ab140175)
  • Western blot - Anti-FBXL5 antibody (ab140175)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FBXL5 antibody (ab140175)

Key features and details

  • Rabbit polyclonal to FBXL5
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-FBXL5 antibody

  • Description

    Rabbit polyclonal to FBXL5

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rat

  • Immunogen

    Recombinant fragment, corresponding to amino acids 510-691 of Human FBXL5 (BC030656)

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Lyophilized:Reconstitute with 200ul distilled sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • Storage buffer

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 1% BSA, 98% PBS
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab140175 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/2000. Predicted molecular weight: 78 kDa.
IHC-P (1)
1/100 - 1/500.
Notes
WB
1/1000 - 1/2000. Predicted molecular weight: 78 kDa.
IHC-P
1/100 - 1/500.

Target

  • Function

    Component of some SCF (SKP1-cullin-F-box) protein ligase complex that plays a central role in iron homeostasis by promoting the ubiquitination and subsequent degradation of IREB2/IRP2. Upon high iron and oxygen level, it specifically recognizes and binds IREB2/IRP2, promoting its ubiquitination and degradation by the proteasome. Promotes ubiquitination and subsequent degradation of DCTN1/p150-glued.

  • Pathway

    Protein modification; protein ubiquitination.

  • Sequence similarities

    Contains 1 F-box domain.
    Contains 7 LRR (leucine-rich) repeats.

  • Domain

    The hemerythrin-like region acts as an oxygen and iron sensor by binding oxygen through a diiron metal-center. In absence of oxygen and iron, the protein is ubiquitinated and degraded.

  • Post-translational
    modifications

    Ubiquitinated upon iron and oxygen depletion, leading to its degradation by the proteasome. Ubiquitination is regulated by the hemerythrin-like region that acts as an oxygen and iron sensor.

  • Cellular localization

    Cytoplasm > perinuclear region.
  • Information by UniProt

  • Database links

    • Alternative names

      • F box and leucine rich repeat protein 5 antibody
      • F box protein FBL5 antibody
      • F box/LRR repeat protein 5 antibody
      • F-box and leucine-rich repeat protein 5 antibody
      • F-box protein FBL4/FBL5 antibody
      • F-box/LRR-repeat protein 5 antibody
      • FBL4 antibody
      • FBL5 antibody
      • FBXL5 antibody
      • FBXL5_HUMAN antibody
      • FLR1 antibody
      • p45SKP2 like protein antibody
      • p45SKP2-like protein antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FBXL5 antibody (ab140175)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FBXL5 antibody (ab140175)This image is courtesy of an anonymous Abreview

      ab140175 staining FBXL5 in Mouse 14.5dpc tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA for 1 hour at room temperature; antigen retrieval was by heat mediation in Tris pH 9. Samples were incubated with primary antibody (1/100 in 1% BSA + 1%FBS in TBS) for 16 hours. An undiluted HRP-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.

      See Abreview

    • Western blot - Anti-FBXL5 antibody (ab140175)
      Western blot - Anti-FBXL5 antibody (ab140175)
      Anti-FBXL5 antibody (ab140175) at 1/1000 dilution + Human fetal testis lysate

      Predicted band size: 78 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FBXL5 antibody (ab140175)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FBXL5 antibody (ab140175)
      Immunohistochemical analysis of formalin-fixed, parafin-embedded Human fetal testis tissue labelling FBXL5 with ab140175 at 1/100 dilution.

    References (1)

    Publishing research using ab140175? Please let us know so that we can cite the reference in this datasheet.

    ab140175 has been referenced in 1 publication.

    • Yao H  et al. F-box and leucine-rich repeat protein 5 promotes colon cancer progression by modulating PTEN/PI3K/AKT signaling pathway. Biomed Pharmacother 107:1712-1719 (2018). PubMed: 30257389

    Customer reviews and Q&As

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    1-2 of 2 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-FBXL5 antibody

     Good
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: r°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris pH 9
    Sample
    Mouse Tissue sections (Mouse 14.5dpc)
    Specification
    Mouse 14.5dpc
    Permeabilization
    No
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Nov 15 2013

    Immunocytochemistry/ Immunofluorescence abreview for Anti-FBXL5 antibody

     Excellent
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
    Sample
    Human Cell (MCF7)
    Specification
    MCF7
    Permeabilization
    Yes - 0,5 Tw-20 in PBS
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Oct 14 2013

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