Anti-FBXL5 antibody (ab140175)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Anti-FBXL5 antibody
See all FBXL5 primary antibodies -
Description
Rabbit polyclonal to FBXL5 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat
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Immunogen
Recombinant fragment, corresponding to amino acids 510-691 of Human FBXL5 (BC030656)
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Positive control
- Human fetal testis tissue.
Properties
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Form
Lyophilised:Reconstitute with 200ul distilled sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary. -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, 98% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab140175 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use at an assay dependent concentration. | |
| WB | 1/1000 - 1/2000. Predicted molecular weight: 78 kDa. | |
| IHC-P | 1/100 - 1/500. |
Target
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Function
Component of some SCF (SKP1-cullin-F-box) protein ligase complex that plays a central role in iron homeostasis by promoting the ubiquitination and subsequent degradation of IREB2/IRP2. Upon high iron and oxygen level, it specifically recognizes and binds IREB2/IRP2, promoting its ubiquitination and degradation by the proteasome. Promotes ubiquitination and subsequent degradation of DCTN1/p150-glued. -
Pathway
Protein modification; protein ubiquitination. -
Sequence similarities
Contains 1 F-box domain.
Contains 7 LRR (leucine-rich) repeats. -
Domain
The hemerythrin-like region acts as an oxygen and iron sensor by binding oxygen through a diiron metal-center. In absence of oxygen and iron, the protein is ubiquitinated and degraded. -
Post-translational
modificationsUbiquitinated upon iron and oxygen depletion, leading to its degradation by the proteasome. Ubiquitination is regulated by the hemerythrin-like region that acts as an oxygen and iron sensor. -
Cellular localization
Cytoplasm > perinuclear region. - Information by UniProt
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Database links
- Entrez Gene: 26234 Human
- Entrez Gene: 242960 Mouse
- Entrez Gene: 305424 Rat
- Omim: 605655 Human
- SwissProt: Q9UKA1 Human
- SwissProt: Q8C2S5 Mouse
- Unigene: 643433 Human
- Unigene: 714386 Human
see all -
Alternative names
- F box and leucine rich repeat protein 5 antibody
- F box protein FBL5 antibody
- F box/LRR repeat protein 5 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FBXL5 antibody (ab140175)This image is courtesy of an anonymous Abreviewab140175 staining FBXL5 in Mouse 14.5dpc tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA for 1 hour at room temperature; antigen retrieval was by heat mediation in Tris pH 9. Samples were incubated with primary antibody (1/100 in 1% BSA + 1%FBS in TBS) for 16 hours. An undiluted HRP-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.
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Immunocytochemistry/ Immunofluorescence - Anti-FBXL5 antibody (ab140175)This image is courtesy of an anonymous Abreviewab140175 staining FBXL5 in MCF7 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized with 0.5% Tween-20 in PBS and blocked with 3% BSA for 1 hour at room temperature. Samples were incubated with primary antibody (1/50 in PBS + 0.02% Tween-20 + 1% BSA) for 1 hour at 27°C. An Alexa Fluor®-conjugated Donkey anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody. Left - nuclei, middle - FBXL5, right - merge.
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Western blot - Anti-FBXL5 antibody (ab140175)Anti-FBXL5 antibody (ab140175) at 1/1000 dilution + Human fetal testis lysate
Predicted band size: 78 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FBXL5 antibody (ab140175)Immunohistochemical analysis of formalin-fixed, parafin-embedded Human fetal testis tissue labelling FBXL5 with ab140175 at 1/100 dilution.
Protocols
References
This product has been referenced in:
- Yao H et al. F-box and leucine-rich repeat protein 5 promotes colon cancer progression by modulating PTEN/PI3K/AKT signaling pathway. Biomed Pharmacother 107:1712-1719 (2018). Read more (PubMed: 30257389) »
