Product nameAnti-FBXL5 antibody
See all FBXL5 primary antibodies
DescriptionMouse polyclonal to FBXL5
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Full length protein, corresponding to amino acids 1-691 of Human FBXL5
- FBXL5 transfected mammalian cell lysate
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.20
Constituent: 2.68% PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab68069 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Detection Antibody Pair.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionComponent of some SCF (SKP1-cullin-F-box) protein ligase complex that plays a central role in iron homeostasis by promoting the ubiquitination and subsequent degradation of IREB2/IRP2. Upon high iron and oxygen level, it specifically recognizes and binds IREB2/IRP2, promoting its ubiquitination and degradation by the proteasome. Promotes ubiquitination and subsequent degradation of DCTN1/p150-glued.
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesContains 1 F-box domain.
Contains 7 LRR (leucine-rich) repeats.
DomainThe hemerythrin-like region acts as an oxygen and iron sensor by binding oxygen through a diiron metal-center. In absence of oxygen and iron, the protein is ubiquitinated and degraded.
modificationsUbiquitinated upon iron and oxygen depletion, leading to its degradation by the proteasome. Ubiquitination is regulated by the hemerythrin-like region that acts as an oxygen and iron sensor.
Cellular localizationCytoplasm > perinuclear region.
- Information by UniProt
- F box and leucine rich repeat protein 5 antibody
- F box protein FBL5 antibody
- F box/LRR repeat protein 5 antibody
All lanes : Anti-FBXL5 antibody (ab68069) at 1/500 dilution
Lane 1 : FBXL5 transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size: 79 kDa
Observed band size: 85 kDa why is the actual band size different from the predicted?
This product has been referenced in:
- Ripa R et al. MicroRNA miR-29 controls a compensatory response to limit neuronal iron accumulation during adult life and aging. BMC Biol 15:9 (2017). Read more (PubMed: 28193224) »
- Chen ZW et al. FBXL5-mediated degradation of single-stranded DNA-binding protein hSSB1 controls DNA damage response. Nucleic Acids Res 42:11560-9 (2014). Read more (PubMed: 25249620) »