Overview

  • Product name

    Anti-FBXO10 antibody
  • Description

    Rabbit polyclonal to FBXO10
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human FBXO10 aa 248-400.
    Database link: Q9UK96

  • Positive control

    • IHC-P: Human liver cancer and prostate tissues. ICC/IF: HepG2 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab236789 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500 - 1/1000.
ICC/IF 1/50 - 1/200.

Target

  • Function

    Substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex.
  • Sequence similarities

    Contains 1 F-box domain.
    Contains 17 PbH1 repeats.
  • Information by UniProt
  • Database links

  • Alternative names

    • F box protein 10 antibody
    • F-box only protein 10 antibody
    • FBX10 antibody
    • FBX10_HUMAN antibody
    • Fbxo10 antibody
    • hCG_2011110 antibody
    • PRMT11 antibody
    see all

Images

  • Paraffin-embedded human liver cancer tissue stained for FBXO10 using ab236789 at 1/500 dilution in immunohistochemical analysis.

    After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • Paraffin-embedded human prostate tissue stained for FBXO10 using ab236789 at 1/500 dilution in immunohistochemical analysis.

    After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • HepG2 (human liver hepatocellular carcinoma cell line) cells stained for FBXO10 (green) using ab236789 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).

References

ab236789 has not yet been referenced specifically in any publications.

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