Product nameAnti-FBXO2 antibody [EPR7328(2)]
See all FBXO2 primary antibodies
DescriptionRabbit monoclonal [EPR7328(2)] to FBXO2
Tested applicationsSuitable for: WB, Flow Cytmore details
Unsuitable for: ICC,IHC-P or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide corresponding to a region within Human FBXO2 (Q9UK22).
- Human fetal brain lysate, NIH/3T3 cells and cell lysate, Neuro2a cell lysate, Human fetal lung lysate, HT29 cell lysate, U87 MG cell lysate.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 2.71 x 10 -10 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab133717 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa).|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionSubstrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex that mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Involved in the endoplasmic reticulum-associated degradation pathway (ERAD) for misfolded lumenal proteins by recognizing and binding sugar chains on unfolded glycoproteins that are retrotranslocated into the cytosol and promoting their ubiquitination and subsequent degradation. Prevents formation of cytosolic aggregates of unfolded glycoproteins that have been retrotranslocated into the cytosol. Able to recognize and bind denatured glycoproteins, preferentially those of the high-mannose type.
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesContains 1 F-box domain.
Contains 1 FBA (F-box associated) domain.
Cellular localizationCytoplasm. Microsome membrane.
- Information by UniProt
- F box gene 1 antibody
- F box only protein 2 antibody
- F box protein 2 antibody
All lanes : Anti-FBXO2 antibody [EPR7328(2)] (ab133717) at 1/10000 dilution
Lane 1 : Human fetal brain lysate
Lane 2 : NIH 3T3 cell lysate
Lane 3 : Neuro 2a cell lysate
Lane 4 : Human fetal lung lysate
Lane 5 : HT29 cell lysate
Lane 6 : U87 MG cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution
Predicted band size: 33 kDa
Flow cytometric analysis of permeabilized NIH 3T3 cells labelling FBXO2 with ab133717 at 1/10 (red) or a rabbit IgG negative control antibody (green).
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
This product has been referenced in:
- Schafer ST et al. Pathological priming causes developmental gene network heterochronicity in autistic subject-derived neurons. Nat Neurosci N/A:N/A (2019). Flow Cyt . Read more (PubMed: 30617258) »
- Liu B et al. Aberrant Expression of Fbxo2 Disrupts Glucose Homeostasis Through Ubiquitin-Mediated Degradation of Insulin Receptor in Obese Mice. Diabetes : (2016). Read more (PubMed: 27932386) »