Product nameAnti-FBXO22 antibody
See all FBXO22 primary antibodies
DescriptionRabbit polyclonal to FBXO22
Tested applicationsSuitable for: WB, ELISAmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Horse, Guinea pig, Cow, Cat, Dog, Zebrafish
- HepG2 cell lysate.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab82974 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 30 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|ELISA||Use at an assay dependent concentration.
Titre using peptide based assay: 1/12500.
FunctionSubstrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex.
Tissue specificityPredominantly expressed in liver.
Sequence similaritiesContains 1 F-box domain.
- Information by UniProt
- 0610033L19Rik antibody
- 1600016C16Rik antibody
- F box only protein 22 antibody
Anti-FBXO22 antibody (ab82974) at 1 µg/ml (in 5% skim milk / PBS buffer) + HepG2 cell lysate at 10 µg
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 30 kDa
Observed band size: 28 kDa why is the actual band size different from the predicted?
Gel concentration: 12%
ab82974 has not yet been referenced specifically in any publications.