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    fbxo28-antibody-ab154068.pdf

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Cell Biology Proteolysis / Ubiquitin Proteasome / Ubiquitin Ubiquitin E3 Enzymes SCF Complex E3 Ligase
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Anti-FBXO28 antibody (ab154068)

  • Datasheet
  • SDS
Submit a review Submit a question References (1)

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Western blot - Anti-FBXO28 antibody (ab154068)
  • Immunocytochemistry/ Immunofluorescence - Anti-FBXO28 antibody (ab154068)

Key features and details

  • Rabbit polyclonal to FBXO28
  • Suitable for: WB, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

You may also be interested in

Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-FBXO28 antibody
    See all FBXO28 primary antibodies
  • Description

    Rabbit polyclonal to FBXO28
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Xenopus laevis, Xenopus tropicalis
  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 91-312 of Human FBXO28 (UniProt: Q9NVF7).

  • Positive control

    • A549 and H1299 whole cell lysates; HeLa cells.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteasome / Ubiquitin
    • Ubiquitin E3 Enzymes
    • SCF Complex E3 Ligase
    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteasome / Ubiquitin
    • Ubiquitin E3 Enzymes
    • F Box E3 Ligase

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • A549 whole cell lysate (ab7910)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab154068 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/500 - 1/3000. Predicted molecular weight: 41 kDa.
ICC/IF
1/100 - 1/1000.
Notes
WB
1/500 - 1/3000. Predicted molecular weight: 41 kDa.
ICC/IF
1/100 - 1/1000.

Target

  • Function

    Probably recognizes and binds to some phosphorylated proteins and promotes their ubiquitination and degradation.
  • Sequence similarities

    Contains 1 F-box domain.
  • Target information above from: UniProt accession Q9NVF7 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 537745 Cow
    • Entrez Gene: 23219 Human
    • Entrez Gene: 67948 Mouse
    • Entrez Gene: 305105 Rat
    • Entrez Gene: 100380991 Xenopus laevis
    • Entrez Gene: 100125127 Xenopus tropicalis
    • Omim: 609100 Human
    • SwissProt: Q2NL16 Cow
    • SwissProt: Q9NVF7 Human
    • SwissProt: Q8BIG4 Mouse
    • Unigene: 64691 Human
    • Unigene: 713992 Human
    • Unigene: 30737 Mouse
    see all
  • Alternative names

    • CENP 30 antibody
    • Centromere protein 30 antibody
    • F box only protein 28 antibody
    • F box protein 28 antibody
    • F-box only protein 28 antibody
    • Fbx28 antibody
    • FBX28_HUMAN antibody
    • FBXO28 antibody
    • FLJ10766 antibody
    • KIAA0483 antibody
    • OTTHUMP00000035597 antibody
    see all

Images

  • Western blot - Anti-FBXO28 antibody (ab154068)
    Western blot - Anti-FBXO28 antibody (ab154068)
    All lanes : Anti-FBXO28 antibody (ab154068) at 1/1000 dilution

    Lane 1 : A549 whole cell lysate
    Lane 2 : H1299 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 41 kDa



    10% SDS PAGE
  • Immunocytochemistry/ Immunofluorescence - Anti-FBXO28 antibody (ab154068)
    Immunocytochemistry/ Immunofluorescence - Anti-FBXO28 antibody (ab154068)
    Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling FBXO28 with ab154068 at 1/500 dilution.
    The lower image has been merged with a DNA probe.

Protocols

  • Western blot protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (1)

Publishing research using ab154068? Please let us know so that we can cite the reference in this datasheet.

ab154068 has been referenced in 1 publication.

  • Gorrepati KDD  et al. An SCFFBXO28 E3 Ligase Protects Pancreatic ß-Cells from Apoptosis. Int J Mol Sci 19:N/A (2018). PubMed: 29587369

Customer reviews and Q&As

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