Anti-FBXO28 antibody (ab154068)
- Datasheet
- Specific References (1)
- Protocols
Overview
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Product nameAnti-FBXO28 antibody
See all FBXO28 primary antibodies -
DescriptionRabbit polyclonal to FBXO28
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Host speciesRabbit
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Tested applicationsSuitable for: WB, ICC/IFmore details
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Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow, Xenopus laevis, Xenopus tropicalis
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Immunogen
Recombinant fragment corresponding to a region within amino acids 91-312 of Human FBXO28 (UniProt: Q9NVF7).
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Positive control
- A549 and H1299 whole cell lysates; HeLa cells.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 0.75% Glycine, 1.21% Tris, 20% Glycerol -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab154068 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 41 kDa. | |
| ICC/IF | 1/100 - 1/1000. |
Target
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FunctionProbably recognizes and binds to some phosphorylated proteins and promotes their ubiquitination and degradation.
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Sequence similaritiesContains 1 F-box domain.
- Information by UniProt
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Database links
- Entrez Gene: 537745 Cow
- Entrez Gene: 23219 Human
- Entrez Gene: 67948 Mouse
- Entrez Gene: 305105 Rat
- Entrez Gene: 100380991 Xenopus laevis
- Entrez Gene: 100125127 Xenopus tropicalis
- Omim: 609100 Human
- SwissProt: Q2NL16 Cow
see all -
Alternative names
- CENP 30 antibody
- Centromere protein 30 antibody
- F box only protein 28 antibody
see all
Images
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Western blot - Anti-FBXO28 antibody (ab154068)All lanes : Anti-FBXO28 antibody (ab154068) at 1/1000 dilution
Lane 1 : A549 whole cell lysate
Lane 2 : H1299 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 41 kDa
10% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-FBXO28 antibody (ab154068)Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling FBXO28 with ab154068 at 1/500 dilution.
The lower image has been merged with a DNA probe.
References
This product has been referenced in:
- Gorrepati KDD et al. An SCFFBXO28 E3 Ligase Protects Pancreatic ß-Cells from Apoptosis. Int J Mol Sci 19:N/A (2018). Read more (PubMed: 29587369) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"