Anti-FBXO28 antibody (ab154068)
Key features and details
- Rabbit polyclonal to FBXO28
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-FBXO28 antibody
See all FBXO28 primary antibodies -
Description
Rabbit polyclonal to FBXO28 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Xenopus laevis, Xenopus tropicalis
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Immunogen
Recombinant fragment corresponding to a region within amino acids 91-312 of Human FBXO28 (UniProt: Q9NVF7).
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Positive control
- A549 and H1299 whole cell lysates; HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab154068 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 41 kDa.
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| ICC/IF |
1/100 - 1/1000.
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| Notes |
|---|
|
WB
1/500 - 1/3000. Predicted molecular weight: 41 kDa. |
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ICC/IF
1/100 - 1/1000. |
Target
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Function
Probably recognizes and binds to some phosphorylated proteins and promotes their ubiquitination and degradation. -
Sequence similarities
Contains 1 F-box domain. - Information by UniProt
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Database links
- Entrez Gene: 537745 Cow
- Entrez Gene: 23219 Human
- Entrez Gene: 67948 Mouse
- Entrez Gene: 305105 Rat
- Entrez Gene: 100380991 Xenopus laevis
- Entrez Gene: 100125127 Xenopus tropicalis
- Omim: 609100 Human
- SwissProt: Q2NL16 Cow
see all -
Alternative names
- CENP 30 antibody
- Centromere protein 30 antibody
- F box only protein 28 antibody
see all
Images
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Western blot - Anti-FBXO28 antibody (ab154068)All lanes : Anti-FBXO28 antibody (ab154068) at 1/1000 dilution
Lane 1 : A549 whole cell lysate
Lane 2 : H1299 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 41 kDa
10% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-FBXO28 antibody (ab154068)Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling FBXO28 with ab154068 at 1/500 dilution.
The lower image has been merged with a DNA probe.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab154068 has been referenced in 1 publication.
- Gorrepati KDD et al. An SCFFBXO28 E3 Ligase Protects Pancreatic ß-Cells from Apoptosis. Int J Mol Sci 19:N/A (2018). PubMed: 29587369