Anti-FBXO31 antibody (ab68067)
Key features and details
- Mouse polyclonal to FBXO31
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-FBXO31 antibody
See all FBXO31 primary antibodies -
Description
Mouse polyclonal to FBXO31 -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein within Human FBXO31 aa 1-400. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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Positive control
- FBXO31 transfected mammalian cell lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.4
Constituent: PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab68067 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/500 - 1/1000. Predicted molecular weight: 66 kDa.
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Notes |
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WB
1/500 - 1/1000. Predicted molecular weight: 66 kDa. |
Target
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Function
Component of some SCF (SKP1-cullin-F-box) protein ligase complex that plays a central role in G1 arrest following DNA damage. Specifically recognizes phosphorylated cyclin-D1 (CCND1), promoting its ubiquitination and degradation by the proteasome, resulting in G1 arrest. May act as a tumor suppressor. -
Tissue specificity
Highly expressed in brain. Expressed at moderate levels in most tissues, except bone marrow. -
Pathway
Protein modification; protein ubiquitination. -
Sequence similarities
Belongs to the FBXO31 family.
Contains 1 F-box domain. -
Developmental stage
Expression is cell-cycle regulated, and peaks at late G2 to early G1 phase (at protein level). -
Post-translational
modificationsPhosphorylation at Ser-278 by ATM following gamma-irradiation results in its stabilization. - Information by UniProt
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Database links
- Entrez Gene: 79791 Human
- Omim: 609102 Human
- SwissProt: Q5XUX0 Human
- Unigene: 567582 Human
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Alternative names
- DKFZp434B027 antibody
- DKFZp434J1815 antibody
- F box only protein 31 antibody
see all
Images
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All lanes : Anti-FBXO31 antibody (ab68067) at 1/500 dilution
Lane 1 : FBXO31 (aa 1-367) transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugated at 1/2500 dilution
Predicted band size: 66 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?
Datasheets and documents
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Datasheet download
References (0)
ab68067 has not yet been referenced specifically in any publications.