Overview

  • Product name

  • Description

    Mouse polyclonal to FBXO31
  • Host species

    Mouse
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Full length protein, corresponding to amino acids 1-367 of Human FBXO31

  • Positive control

    • FBXO31 transfected mammalian cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab68067 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 66 kDa.

Target

  • Function

    Component of some SCF (SKP1-cullin-F-box) protein ligase complex that plays a central role in G1 arrest following DNA damage. Specifically recognizes phosphorylated cyclin-D1 (CCND1), promoting its ubiquitination and degradation by the proteasome, resulting in G1 arrest. May act as a tumor suppressor.
  • Tissue specificity

    Highly expressed in brain. Expressed at moderate levels in most tissues, except bone marrow.
  • Pathway

    Protein modification; protein ubiquitination.
  • Sequence similarities

    Belongs to the FBXO31 family.
    Contains 1 F-box domain.
  • Developmental stage

    Expression is cell-cycle regulated, and peaks at late G2 to early G1 phase (at protein level).
  • Post-translational
    modifications

    Phosphorylation at Ser-278 by ATM following gamma-irradiation results in its stabilization.
  • Information by UniProt
  • Database links

  • Alternative names

    • DKFZp434B027 antibody
    • DKFZp434J1815 antibody
    • F box only protein 31 antibody
    • F box protein 31 antibody
    • F-box only protein 31 antibody
    • FBX14 antibody
    • FBX31 antibody
    • FBX31_HUMAN antibody
    • FBXO14 antibody
    • Fbxo31 antibody
    • FLJ22477 antibody
    • MGC15419 antibody
    • MGC9527 antibody
    • pp2386 antibody
    • Putative breast cancer tumor suppressor antibody
    • SCF ubiquitin ligase specificity factor antibody
    see all

Images

  • All lanes : Anti-FBXO31 antibody (ab68067) at 1/500 dilution

    Lane 1 : FBXO31 (aa 1-367) transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugated at 1/2500 dilution

    Predicted band size: 66 kDa
    Observed band size: 40 kDa
    why is the actual band size different from the predicted?

References

ab68067 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Application
Western blot
Sample
Human Cell lysate - whole cell (HT29, HCT116 (human colon cancer))
Gel Running Conditions
Reduced Denaturing (10% SDS-PAGE)
Loading amount
12 µg
Specification
HT29, HCT116 (human colon cancer)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Oct 30 2018

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (MDA-MB-231 cell line)
Permeabilization
Yes - Tween-20
Specification
MDA-MB-231 cell line
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative
Acetone

Abcam user community

Verified customer

Submitted Oct 29 2018

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Tumor tissue (from K562 cell xenograft mouse))
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: pH 6.0 Citric Acid
Permeabilization
Yes - Tween-20
Specification
Tumor tissue (from K562 cell xenograft mouse)
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Oct 29 2018

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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