Key features and details
- Mouse polyclonal to FBXO31
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-FBXO31 antibody
See all FBXO31 primary antibodies
DescriptionMouse polyclonal to FBXO31
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Full length protein, corresponding to amino acids 1-367 of Human FBXO31
- FBXO31 transfected mammalian cell lysate
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.4
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab68067 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 66 kDa.|
FunctionComponent of some SCF (SKP1-cullin-F-box) protein ligase complex that plays a central role in G1 arrest following DNA damage. Specifically recognizes phosphorylated cyclin-D1 (CCND1), promoting its ubiquitination and degradation by the proteasome, resulting in G1 arrest. May act as a tumor suppressor.
Tissue specificityHighly expressed in brain. Expressed at moderate levels in most tissues, except bone marrow.
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesBelongs to the FBXO31 family.
Contains 1 F-box domain.
Developmental stageExpression is cell-cycle regulated, and peaks at late G2 to early G1 phase (at protein level).
modificationsPhosphorylation at Ser-278 by ATM following gamma-irradiation results in its stabilization.
- Information by UniProt
- DKFZp434B027 antibody
- DKFZp434J1815 antibody
- F box only protein 31 antibody
All lanes : Anti-FBXO31 antibody (ab68067) at 1/500 dilution
Lane 1 : FBXO31 (aa 1-367) transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugated at 1/2500 dilution
Predicted band size: 66 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?
ab68067 has not yet been referenced specifically in any publications.