Key features and details
- Rabbit polyclonal to FBXO38
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-FBXO38 antibody
DescriptionRabbit polyclonal to FBXO38
Tested applicationsSuitable for: WB, IP, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Horse, Cow, Dog, Chimpanzee, Rhesus monkey, Gorilla, Opossum, Orangutan, Elephant
Synthetic peptide corresponding to Human FBXO38 aa 852-875.
Database link: NP_995308.1
- HeLa and NIH3T3 whole cell lysates.
This product was previously labelled as F box protein 38
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.80
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab87729 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 134 kDa.|
|IP||Use at 5-10 µg/mg of lysate.|
|IHC-P||1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
RelevanceF box protein 38 probably recognizes and binds to some phosphorylated proteins and promotes their ubiquitination and degradation. It may coactivate KLF7, but does not seem to promote KLF7 ubiquitination. There are three named isoforms.
Cellular localizationCytoplasmic and Nuclear
- F box only protein 38 antibody
- F box protein 38 antibody
- Fbx38 antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma (right) and human testicular seminoma (right) tissues labelling FBXO38 with ab87729 at 1/1000 (1µg/ml). Detection: DAB.
All lanes : Anti-FBXO38 antibody (ab87729) at 0.1 µg/ml
Lane 1 : Whole cell
lysate from HeLa cells at 50 µg
Lane 2 : Whole cell
lysate from HeLa cells at 15 µg
Lane 3 : Whole cell
lysate from HeLa cells at 5 µg
Lane 4 : Whole cell
lysate from NIH3T3 cells at 50 µg
Developed using the ECL technique.
Predicted band size: 134 kDa
Observed band size: 134 kDa
Additional bands at: 107 kDa (possible isoform)
Exposure time: 3 minutes
Detection of FBXO38 in Immunoprecipitates of Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab87729 at 10 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 30 seconds.
ab87729 has not yet been referenced specifically in any publications.