Overview

  • Product name

    Anti-FBXO38 antibody
  • Description

    Rabbit polyclonal to FBXO38
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Horse, Cow, Dog, Chimpanzee, Rhesus monkey, Gorilla, Opossum, Orangutan, Elephant
  • Immunogen

    Synthetic peptide corresponding to Human FBXO38 aa 852-875.
    Database link: NP_995308.1

  • Positive control

    • HeLa and NIH3T3 whole cell lysates.
  • General notes

     This product was previously labelled as F box protein 38

     

Properties

Applications

Our Abpromise guarantee covers the use of ab87729 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 134 kDa.
IP Use at 5-10 µg/mg of lysate.
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma (right) and human testicular seminoma (right) tissues labelling FBXO38 with ab87729 at 1/1000 (1µg/ml). Detection: DAB.

  • All lanes : Anti-FBXO38 antibody (ab87729) at 0.1 µg/ml

    Lane 1 : Whole cell
    lysate from HeLa cells at 50 µg
    Lane 2 : Whole cell
    lysate from HeLa cells at 15 µg
    Lane 3 : Whole cell
    lysate from HeLa cells at 5 µg
    Lane 4 : Whole cell
    lysate from NIH3T3 cells at 50 µg

    Developed using the ECL technique.

    Predicted band size: 134 kDa
    Observed band size: 134 kDa
    Additional bands at: 107 kDa (possible isoform)


    Exposure time: 3 minutes
  • Detection of FBXO38 in Immunoprecipitates of Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab87729 at 10 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 30 seconds.

References

ab87729 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

Thank you very much for your interest in Abcam anti-F box protein 38 antibodies.

Because we carry over 75,000 products, it isn't feasible for us to keep small sample sizes of our products.

We are happy to reassure our customers that all of our products are covered by our Abpromise, which guarantees that the product will work in the applications and species specified on the datasheet, or we will offer a replacement, credit, or refund within 6 months of purchase. The antibody ab87729 is covered by our guarantee for a use in IP. https://www.abcam.com/ab87729 .

If a product is to be used in an untested species or application, such as ab67855 to be used in IP(https://www.abcam.com/ab67855), I can offer a discount off a future purchase if you buy ab67855 now, test it in IP and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody.

If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and test ab67855 in IP. I will then send a discount code. This code must be issued before purchasing ab67855 so please wait for my reply before ordering.

2. Purchase ab67855 either by phone, fax, or online (https://www.abcam.com).

3. Test it in IP.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for anyprimary antibodyordered and the discount code is valid for 4 months after issue.
Please remember that submission of the Abreview is sufficient for the discount code to become active.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab67855 turns out to be unsuitable for IP, you will still receive the discount on your next purchase after your Abreview has been submitted.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: https://www.abcam.com/collaborationdiscount.

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