Overview

  • Product name

  • Description

    Rabbit polyclonal to FBXO4/FBX4
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Recombinant fragment within Human FBXO4/FBX4 (internal sequence). The exact sequence is proprietary.
    Database link: Q9UKT5

  • Positive control

    • ICC/IF: HeLa cells. WB: A431 and HeLa whole cell lysates.
  • General notes

     This product was previously labelled as FBXO4

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab227442 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 44 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex that mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Promotes ubiquitination of CCND1 and its subsequent proteasomal degradation. Recognizes TERF1 and promotes its ubiquitination together with UBE2D1.
  • Pathway

    Protein modification; protein ubiquitination.
  • Sequence similarities

    Contains 1 F-box domain.
  • Post-translational
    modifications

    Phosphorylation at Ser-12 varies during the cell cycle. It is low in resting cells and high in the S phase and the G2/M phase of the cell cycle. Phosphorylation is decreased during late G1 phase (By similarity). Phosphorylation at Ser-12 promotes homodimerization and is necessary for optimal ubiquitin ligase activity towards CCND1.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • DKFZp547N213 antibody
    • F box only protein 4 antibody
    • F box protein 4 antibody
    • F box protein Fbx4 antibody
    • F-box only protein 4 antibody
    • FBX4 antibody
    • FBX4_HUMAN antibody
    • FBXO 4 antibody
    • FBXO4 antibody
    • FLJ10141 antibody
    see all

Images

  • Methanol-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for FBXO4/FBX4 (green) using ab227442 at 1/500 dilution in ICC/IF. Counterstain: Alpha-tubulin filaments were labeled with an alpha-tubulin antibody at 1/2000 dilution (red).

  • All lanes : Anti-FBXO4/FBX4 antibody (ab227442) at 1/2000 dilution

    Lane 1 : A431 (human epidermoid carcinoma cell line) whole cell lysate
    Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 44 kDa



    10% SDS-PAGE gel.

References

ab227442 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab227442.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up