Key features and details
- Rabbit polyclonal to FBXO4/FBX4
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-FBXO4/FBX4 antibody
See all FBXO4/FBX4 primary antibodies
DescriptionRabbit polyclonal to FBXO4/FBX4
Tested applicationsSuitable for: WB, IP, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Cow
Recombinant fragment corresponding to Human FBXO4/FBX4 aa 138-387.
DYMAVYRMCCPYTRRASKSSRPMYGAVTSFLHSLIIQNEPRFAMFGPGLE ELNTSLVLSLMSSEELCPTAGLPQRQIDGIGSGVNFQLNNQHKFNILILY STTRKERDRAREEHTSAVNKMFSRHNEGDDQQGSRYSVIPQIQKVCEVVD GFIYVANAEAHKRHEWQDEFSHIMAMTDPAFGSSGRPLLVLSCISQGDVK RMPCFYLAHELHLNLLNHPWLVQDTEAETLTGFLNGIEWILEEVESKRAR
Database link: Q9UKT5
- WB: HEK-293T, HepG2 and HL-60 whole cell lysate. IP: FBXO4/FBX4 IP in HepG2 whole cell lysate. IHC-P: Human brain and heart tissue.
This product was previously labelled as FBXO4
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab230302 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Detects a band of approximately 44 kDa (predicted molecular weight: 44, 35 kDa).|
|IP||1/200 - 1/2000.|
|IHC-P||1/20 - 1/200.|
FunctionSubstrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex that mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Promotes ubiquitination of CCND1 and its subsequent proteasomal degradation. Recognizes TERF1 and promotes its ubiquitination together with UBE2D1.
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesContains 1 F-box domain.
modificationsPhosphorylation at Ser-12 varies during the cell cycle. It is low in resting cells and high in the S phase and the G2/M phase of the cell cycle. Phosphorylation is decreased during late G1 phase (By similarity). Phosphorylation at Ser-12 promotes homodimerization and is necessary for optimal ubiquitin ligase activity towards CCND1.
- Information by UniProt
- DKFZp547N213 antibody
- F box only protein 4 antibody
- F box protein 4 antibody
All lanes : Anti-FBXO4/FBX4 antibody (ab230302) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 3 : HL-60 human promyelocytic leukemia cell line) whole cell lysate
All lanes : Goat polyclonal to rabbit IgG at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 44, 35 kDa
Observed band size: 44 kDa why is the actual band size different from the predicted?
Paraffin-embedded human brain tissue stained for FBXO4/FBX4 with ab230302 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human heart tissue stained for FBXO4/FBX4 with ab230302 at 1/100 dilution in immunohistochemical analysis.
FBXO4/FBX4 was immunoprecipitated from 0.5 mg of HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate with ab230302.
HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG, was used as secondary antibody at 1/50,000 dilution.
Lane 1: Rabbit monoclonal IgG (1 μg) instead of ab230302 in HepG2 whole cell lysate.
Lane 2: ab230302 IP in HepG2 whole cell lysate.
Lane 3: HepG2 whole cell lysate 20 μg (Input).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab230302 has not yet been referenced specifically in any publications.