Product nameAnti-FBXO4/FBX4 antibody
See all FBXO4/FBX4 primary antibodies
DescriptionRabbit polyclonal to FBXO4/FBX4
Tested applicationsSuitable for: WB, IP, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Cow
Recombinant fragment corresponding to Human FBXO4/FBX4 aa 138-387.
DYMAVYRMCCPYTRRASKSSRPMYGAVTSFLHSLIIQNEPRFAMFGPGLE ELNTSLVLSLMSSEELCPTAGLPQRQIDGIGSGVNFQLNNQHKFNILILY STTRKERDRAREEHTSAVNKMFSRHNEGDDQQGSRYSVIPQIQKVCEVVD GFIYVANAEAHKRHEWQDEFSHIMAMTDPAFGSSGRPLLVLSCISQGDVK RMPCFYLAHELHLNLLNHPWLVQDTEAETLTGFLNGIEWILEEVESKRAR
Database link: Q9UKT5
- WB: HEK-293T, HepG2 and HL-60 whole cell lysate. IP: FBXO4/FBX4 IP in HepG2 whole cell lysate. IHC-P: Human brain and heart tissue.
This product was previously labelled as FBXO4
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab230302 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Detects a band of approximately 44 kDa (predicted molecular weight: 44, 35 kDa).|
|IP||1/200 - 1/2000.|
|IHC-P||1/20 - 1/200.|
FunctionSubstrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex that mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Promotes ubiquitination of CCND1 and its subsequent proteasomal degradation. Recognizes TERF1 and promotes its ubiquitination together with UBE2D1.
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesContains 1 F-box domain.
modificationsPhosphorylation at Ser-12 varies during the cell cycle. It is low in resting cells and high in the S phase and the G2/M phase of the cell cycle. Phosphorylation is decreased during late G1 phase (By similarity). Phosphorylation at Ser-12 promotes homodimerization and is necessary for optimal ubiquitin ligase activity towards CCND1.
- Information by UniProt
- DKFZp547N213 antibody
- F box only protein 4 antibody
- F box protein 4 antibody
All lanes : Anti-FBXO4/FBX4 antibody (ab230302) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 3 : HL-60 human promyelocytic leukemia cell line) whole cell lysate
All lanes : Goat polyclonal to rabbit IgG at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 44, 35 kDa
Observed band size: 44 kDa why is the actual band size different from the predicted?
Paraffin-embedded human brain tissue stained for FBXO4/FBX4 with ab230302 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human heart tissue stained for FBXO4/FBX4 with ab230302 at 1/100 dilution in immunohistochemical analysis.
FBXO4/FBX4 was immunoprecipitated from 0.5 mg of HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate with ab230302.
HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG, was used as secondary antibody at 1/50,000 dilution.
Lane 1: Rabbit monoclonal IgG (1 μg) instead of ab230302 in HepG2 whole cell lysate.
Lane 2: ab230302 IP in HepG2 whole cell lysate.
Lane 3: HepG2 whole cell lysate 20 μg (Input).
ab230302 has not yet been referenced specifically in any publications.