- Datasheet
- Specific References
- Protocols
Overview
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Product nameAnti-FbxO6 antibody
See all FbxO6 primary antibodies -
DescriptionRabbit polyclonal to FbxO6
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Host speciesRabbit
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Tested applicationsSuitable for: WB, IHC-Pmore details
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Species reactivityReacts with: Human
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Immunogen
Recombinant fragment corresponding to a region within amino acids 48 and 293 of Human FBxO6 (Uniprot ID: Q9NRD1)
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Positive control
- 293T whole cell lysate.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 0.75% Glycine, 1.21% Tris, 20% Glycerol -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Positive Controls
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Related Products
Applications
Our Abpromise guarantee covers the use of ab153853 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 34 kDa. | |
| IHC-P | 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Alternatively Tris-EDTA buffer (pH8.0) may be used. |
Target
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FunctionSubstrate-recognition component of some SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complexes. Involved in endoplasmic reticulum-associated degradation pathway (ERAD) for misfolded lumenal proteins by recognizing and binding sugar chains on unfolded glycoproteins that are retrotranlocated into the cytosol and promoting their ubiquitination and subsequent degradation. Able to recognize and bind denatured glycoproteins, which are modified with not only high-mannose but also complex-type oligosaccharides. Also recognizes sulfated glycans. Also involved in DNA damage response by specifically recognizing activated CHEK1 (phosphorylated on 'Ser-345'), promoting its ubiquitination and degradation. Ubiquitination of CHEK1 is required to insure that activated CHEK1 does not accumulate as cells progress through S phase, or when replication forks encounter transient impediments during normal DNA replication.
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PathwayProtein modification; protein ubiquitination.
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Sequence similaritiesContains 1 F-box domain.
Contains 1 FBA (F-box associated) domain. -
Cellular localizationCytoplasm.
- Information by UniProt
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Database links
- Entrez Gene: 26270 Human
- Omim: 605647 Human
- SwissProt: Q9NRD1 Human
- Unigene: 464419 Human
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Alternative names
- F box protein that recognizes sugar chains 2 antibody
- F box G domain protein 2 antibody
- F box only protein 6 antibody
see all
Images
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Western blot - Anti-FbxO6 antibody (ab153853)Anti-FbxO6 antibody (ab153853) at 1/500 dilution + 293T whole cell lysate at 30 µg
Predicted band size: 34 kDa
12% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FbxO6 antibody (ab153853)Immunohistochemical analysis of paraffin-embedded Colon using ab153853 antibody at 1/500 dilution
References
ab153853 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"