Anti-FbxW2 antibody (ab5309)

Product name

Anti-FbxW2 antibody
See all FbxW2 primary antibodies
Description

Goat polyclonal to FbxW2
Host species

Goat
Tested applications

Suitable for: IHC-P, WBmore details
Species reactivity

Reacts with: Mouse, Rat, Human
Predicted to work with: Cow, Dog, Pig
Immunogen

Synthetic peptide: KRGSSFLAGEHPG, corresponding to C terminal amino acids 410-422 of Human FbxW2.

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Positive control
- Lysate prepared from human kidney. Human kidney tissue sections.

Form

Liquid
Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer

pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% Tris buffered saline, 0.5% BSA
Concentration information loading...
Purity

Immunogen affinity purified
Purification notes

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality

Polyclonal
Isotype

IgG
Research areas

Our Abpromise guarantee covers the use of ab5309 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
IHC-P		Use a concentration of 2 - 3 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB		Use a concentration of 0.3 - 1.5 µg/ml. Detects a band of approximately 30, 48 kDa (predicted molecular weight: 48 kDa).Can be blocked with Human FbxW2 peptide (ab23101). The stronger band at 30kDa was consistently observed, but it is successfully blocked by incubation with the immunising peptide (as is the 48kDa band).

Function

Substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex.
Sequence similarities

Contains 1 F-box domain.
Contains 4 WD repeats.
Target information above from: UniProt accession Q9UKT8 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 26190 Human
- Entrez Gene: 30050 Mouse
- Entrez Gene: 311881 Rat
- Omim: 609071 Human
- SwissProt: Q9UKT8 Human
- SwissProt: Q60584 Mouse
- SwissProt: B2RZ17 Rat
- Unigene: 494985 Human
- Unigene: 4465 Mouse
- Unigene: 103250 Rat
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Alternative names
- F box and WD 40 domain containing protein 2 antibody
- F box and WD repeat domain containing 2 antibody
- F box and WD40 domain protein 2 antibody
- F box/WD repeat containing protein 2 antibody
- F-box and WD-40 domain-containing protein 2 antibody
- F-box/WD repeat-containing protein 2 antibody
- FBW 2 antibody
- FBW2 antibody
- FBXW 2 antibody
- Fbxw2 antibody
- FBXW2_HUMAN antibody
- Fwd2 antibody
- Md6 antibody
- Protein MD6 antibody
see all

Western blot - Anti-FbxW2 antibody (ab5309)

Anti-FbxW2 antibody (ab5309) at 0.3 µg/ml + Human Kidney lysate (RIPA buffer, 30µg total protein per lane).

Predicted band size: 48 kDa

Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FbxW2 antibody (ab5309)

Ab5309 (3ug/ml) staining human FbxW2 in human kidney by immunohistochemistry using paraffin embedded tissue.
Microwaved antigen retrieval with citrate buffer, pH6, HRP-staining.

ab5309 has not yet been referenced specifically in any publications.

Publishing research using ab5309? Please let us know so that we can cite the reference in this datasheet.

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