Anti-FbxW2 antibody (ab5309)
- Datasheet
- References (1)
- Protocols
Overview
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Product nameAnti-FbxW2 antibody
See all FbxW2 primary antibodies -
DescriptionGoat polyclonal to FbxW2
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Host speciesGoat
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Tested applicationsSuitable for: IHC-P, WBmore details
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Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Cow, Dog, Pig
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Immunogen
Synthetic peptide: KRGSSFLAGEHPG, corresponding to C terminal amino acids 410-422 of Human FbxW2.
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Positive control
- Lysate prepared from human kidney. Human kidney tissue sections.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
PurityImmunogen affinity purified
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Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab5309 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use a concentration of 2 - 3 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| WB | Use a concentration of 0.3 - 1.5 µg/ml. Detects a band of approximately 30, 48 kDa (predicted molecular weight: 48 kDa).Can be blocked with Human FbxW2 peptide (ab23101). The stronger band at 30kDa was consistently observed, but it is successfully blocked by incubation with the immunising peptide (as is the 48kDa band). |
Target
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FunctionSubstrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex.
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Sequence similaritiesContains 1 F-box domain.
Contains 4 WD repeats. - Information by UniProt
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Database links
- Entrez Gene: 26190 Human
- Entrez Gene: 30050 Mouse
- Entrez Gene: 311881 Rat
- Omim: 609071 Human
- SwissProt: Q9UKT8 Human
- SwissProt: Q60584 Mouse
- SwissProt: B2RZ17 Rat
- Unigene: 494985 Human
see all -
Alternative names
- F box and WD 40 domain containing protein 2 antibody
- F box and WD repeat domain containing 2 antibody
- F box and WD40 domain protein 2 antibody
see all
Images
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Western blot - Anti-FbxW2 antibody (ab5309)Anti-FbxW2 antibody (ab5309) at 0.3 µg/ml + Human Kidney lysate (RIPA buffer, 30µg total protein per lane).
Predicted band size: 48 kDa
Primary incubated for 1 hour. Detected by western blot using chemiluminescence. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FbxW2 antibody (ab5309)Ab5309 (3ug/ml) staining human FbxW2 in human kidney by immunohistochemistry using paraffin embedded tissue.Microwaved antigen retrieval with citrate buffer, pH6, HRP-staining.
Datasheets and documents
References
This product has been referenced in:
- Akiyama M FBXW2 localizes with osteocalcin in bovine periosteum on culture dishes as visualized by double immunostaining. Heliyon 4:e00782 (2018). IHC-P ; Cow . Read more (PubMed: 30229138) »
