Overview

  • Product name
  • Description
    Rabbit polyclonal to FBXW5
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to a region within internal sequence amino acids 177-432 of Human FBXW5

  • Positive control
    • HeLa whole cell lysate, paraffin-embedded Colon carcinoma tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab154347 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 64 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Relevance
    FBXW5 is a member of the F-box protein family, members of which are characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into three classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. FBXW5 contains WD-40 domains, in addition to an F-box motif, so it belongs to the Fbw class. Alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene, however, they were found to be nonsense-mediated mRNA decay (NMD) candidates, hence not represented.
  • Cellular localization
    Cytoplasmic
  • Database links
  • Alternative names
    • F box and WD 40 domain containing protein 5 antibody
    • F box and WD repeat domain containing 5 antibody
    • F box WD repeat containing protein 5 antibody
    • F box/WD repeat containing protein 5 antibody
    • F-box/WD repeat-containing protein 5 antibody
    • FBW5 antibody
    • FBXW5 antibody
    • WD repeat containing F box protein FBW5 antibody
    see all

Images

  • Anti-FBXW5 antibody (ab154347) at 1/1000 dilution + HeLa whole cell lysate at 30 µg

    Predicted band size: 64 kDa



    7.5% SDS PAGE
  • Immunohistochemical analysis of paraffin-embedded Human colon carcinoma labeling FBXW5 with ab154347 at 1/500 dilution

References

ab154347 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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