• Product name

  • Description

    Rabbit polyclonal to Fbxw7
  • Host species

  • Tested applications

    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Caenorhabditis elegans, Drosophila melanogaster, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to Human Fbxw7 aa 533-582 (C terminal). Synthetic peptide corresponding to a region within C terminal amino acids 533-582 (LKTGEFIRNL VTLESGGSGG VVWRIRASNT KLVCAVGSRN GTEETKLLVL) of Human Fbxw7 (NP_001013433; UniProt ID Q969H0-4 isoform 3).


    Database link: Q969H0-4

  • Positive control

    • Jurkat cell lysate This antibody gave a positive result when used in the following formaldehyde fixed cell lines: HepG2.



Our Abpromise guarantee covers the use of ab105752 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
IHC-P Use a concentration of 2.5 µg/ml.
WB Use a concentration of 0.25 µg/ml. Predicted molecular weight: for isoform 4, 66 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.


  • Function

    Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Probably recognizes and binds to phosphorylated target proteins. Involved in the degradation of cyclin-E, MYC, NOTCH1 released notch intracellular domain (NICD), and probably PSEN1.
  • Tissue specificity

    Isoform 1 is widely expressed. Isoform 4 is expressed in brain.
  • Sequence similarities

    Contains 1 F-box domain.
    Contains 7 WD repeats.
  • Post-translational

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Cytoplasm; Nucleus; Nucleus > nucleolus and Nucleus > nucleoplasm.
  • Information by UniProt
  • Database links

    see all
  • Alternative names

    • AGO antibody
    • Archipelago antibody
    • Archipelago homolog antibody
    • Archipelago, Drosophila, homolog of antibody
    • CDC4 antibody
    • CDC4, S.cerevisiae, homolog of antibody
    • DKFZp686F23254 antibody
    • F box and WD 40 domain protein 7 (archipelago homolog, Drosophila) antibody
    • F box and WD 40 domain protein 7 antibody
    • F box and WD repeat domain containing 7 antibody
    • F box protein FBW7 antibody
    • F box protein FBX30 antibody
    • F box protein SEL 10 antibody
    • F box protein SEL10 antibody
    • F-box and WD repeat domain containing 7, E3 ubiquitin protein ligase antibody
    • F-box and WD-40 domain-containing protein 7 antibody
    • F-box protein FBX30 antibody
    • F-box/WD repeat-containing protein 7 antibody
    • FBW6 antibody
    • FBW7 antibody
    • FBX30 antibody
    • FBXO30 antibody
    • FBXW6 antibody
    • FBXW7 antibody
    • FBXW7_HUMAN antibody
    • FLJ16457 antibody
    • hAgo antibody
    • hCdc4 antibody
    • Homolog of C elegans sel 10 antibody
    • Homolog of C.elegans sel10 antibody
    • SEL 10 antibody
    • SEL-10 antibody
    • SEL10 antibody
    see all


  • Anti-Fbxw7 antibody (ab105752) at 0.25 µg/ml + Jurkat cell lysate at 10 µg

    Predicted band size: for isoform 4, 66 kDa

    Gel concentration: 12%
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human pineal tissue labelling Fbxw7 with ab105752 at 1/100. A Cy3-conjugated donkey anti-rabbit IgG (1/200) was used as the secondary antibody. Positive staining shown in the cytoplasm of cell bodies and processes of pinealocytes. Magnification: 20X. Exposure time: 0.5 - 2.0 seconds. Left - DAPI. Middle - Fbxw7. Right - Merge.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin embedded sections) analysis of human kidney tissue sections labeling Fbxw7 with ab105752 at 2.5 µg/ml.

  • ICC/IF image of ab105752 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab105752 at 10µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Western blot analysis of 721_B whole cell lysate labeling Fbxw7 with ab105752 at 1.0µg/ml.


This product has been referenced in:

  • Tang X  et al. MicroRNA-223 promotes hepatocellular carcinoma cell resistance to sorafenib by targeting FBW7. Oncol Rep 41:1231-1237 (2019). Read more (PubMed: 30535501) »
  • Peng G  et al. miR-25-3p promotes glioma cell proliferation and migration by targeting FBXW7 and DKK3. Exp Ther Med 18:769-778 (2019). Read more (PubMed: 31258712) »
See all 5 Publications for this product

Customer reviews and Q&As

Human Cell lysate - whole cell (cell)
Total protein in input
30 µg
Immuno-precipitation step
Protein G

Abcam user community

Verified customer

Submitted Aug 13 2015

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