Key features and details
- Rabbit polyclonal to Fbxw7
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-Fbxw7 antibody
See all Fbxw7 primary antibodies
DescriptionRabbit polyclonal to Fbxw7
Specificityab192328 detects endogenous levels of Fbxw7 protein.
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
Recombinant full length protein corresponding to Human Fbxw7.
Database link: Q969H0
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.2% Sodium azide
Constituents: 49% PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab192327 is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Our Abpromise guarantee covers the use of ab192328 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 80 kDa.|
|ICC/IF||1/50 - 1/2000.|
FunctionSubstrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Probably recognizes and binds to phosphorylated target proteins. Involved in the degradation of cyclin-E, MYC, NOTCH1 released notch intracellular domain (NICD), and probably PSEN1.
Tissue specificityIsoform 1 is widely expressed. Isoform 4 is expressed in brain.
Sequence similaritiesContains 1 F-box domain.
Contains 7 WD repeats.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationCytoplasm; Nucleus; Nucleus > nucleolus and Nucleus > nucleoplasm.
- Information by UniProt
- AGO antibody
- Archipelago antibody
- Archipelago homolog antibody
ab192328 (1/50) staining FBXW7 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
All lanes : Anti-Fbxw7 antibody (ab192328) at 1/1000 dilution
Lane 1 : Mouse spleen tissue lysate
Lane 2 : Rat lung tissue lysate
Lane 3 : HepG2 cell lysates
Predicted band size: 80 kDa
ab192328 has been referenced in 2 publications.
- Mo SJ et al. Regulation of Fn14 stability by SCFFbxw7a during septic acute kidney injury. Am J Physiol Renal Physiol 316:F1273-F1281 (2019). PubMed: 31017010
- Jiang W et al. Tongxinluo inhibits neointimal formation by regulating the expression and post-translational modification of KLF5 in macrophages. Am J Transl Res 8:4778-4790 (2016). WB ; Mouse . PubMed: 27904679