Overview

  • Product name

  • Description

    Rabbit polyclonal to Fbxw7
  • Host species

    Rabbit
  • Specificity

    ab192328 detects endogenous levels of Fbxw7 protein.
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant full length protein corresponding to Human Fbxw7.
    Database link: Q969H0

  • Positive control

    • WB: Mouse spleen and Rat lung tissue lysates, HepG2 cell lysate ICC/IF: HUVEC cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab192328 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/200.
WB 1/500 - 1/1000. Predicted molecular weight: 80 kDa.
ICC/IF 1/50 - 1/2000.

Target

  • Function

    Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Probably recognizes and binds to phosphorylated target proteins. Involved in the degradation of cyclin-E, MYC, NOTCH1 released notch intracellular domain (NICD), and probably PSEN1.
  • Tissue specificity

    Isoform 1 is widely expressed. Isoform 4 is expressed in brain.
  • Sequence similarities

    Contains 1 F-box domain.
    Contains 7 WD repeats.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Cytoplasm; Nucleus; Nucleus > nucleolus and Nucleus > nucleoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • AGO antibody
    • Archipelago antibody
    • Archipelago homolog antibody
    • Archipelago, Drosophila, homolog of antibody
    • CDC4 antibody
    • CDC4, S.cerevisiae, homolog of antibody
    • DKFZp686F23254 antibody
    • F box and WD 40 domain protein 7 (archipelago homolog, Drosophila) antibody
    • F box and WD 40 domain protein 7 antibody
    • F box and WD repeat domain containing 7 antibody
    • F box protein FBW7 antibody
    • F box protein FBX30 antibody
    • F box protein SEL 10 antibody
    • F box protein SEL10 antibody
    • F-box and WD repeat domain containing 7, E3 ubiquitin protein ligase antibody
    • F-box and WD-40 domain-containing protein 7 antibody
    • F-box protein FBX30 antibody
    • F-box/WD repeat-containing protein 7 antibody
    • FBW6 antibody
    • FBW7 antibody
    • FBX30 antibody
    • FBXO30 antibody
    • FBXW6 antibody
    • FBXW7 antibody
    • FBXW7_HUMAN antibody
    • FLJ16457 antibody
    • hAgo antibody
    • hCdc4 antibody
    • Homolog of C elegans sel 10 antibody
    • Homolog of C.elegans sel10 antibody
    • SEL 10 antibody
    • SEL-10 antibody
    • SEL10 antibody
    see all

Images

  • ab192328 (1/50) staining FBXW7 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • All lanes : Anti-Fbxw7 antibody (ab192328) at 1/1000 dilution

    Lane 1 : Mouse spleen tissue lysate
    Lane 2 : Rat lung tissue lysate
    Lane 3 : HepG2 cell lysates

    Predicted band size: 80 kDa

References

This product has been referenced in:

  • Mo SJ  et al. Regulation of Fn14 stability by SCFFbxw7a during septic acute kidney injury. Am J Physiol Renal Physiol 316:F1273-F1281 (2019). Read more (PubMed: 31017010) »
  • Jiang W  et al. Tongxinluo inhibits neointimal formation by regulating the expression and post-translational modification of KLF5 in macrophages. Am J Transl Res 8:4778-4790 (2016). WB ; Mouse . Read more (PubMed: 27904679) »
See all 2 Publications for this product

Customer reviews and Q&As

Abreviews
Application
Western blot
Sample
Mouse Cell lysate - whole cell (B lymphocytes)
Gel Running Conditions
Reduced Denaturing
Loading amount
10 µg
Specification
B lymphocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Jul 03 2019

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