Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP237] to Fbxw7
- Suitable for: Flow Cyt, IHC-P
- Reacts with: Human
Product nameAnti-Fbxw7 antibody [SP237]
See all Fbxw7 primary antibodies
DescriptionRabbit monoclonal [SP237] to Fbxw7
Tested applicationsSuitable for: Flow Cyt, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide within Human Fbxw7 aa 200-300 (internal sequence). The exact sequence is proprietary.
Database link: Q969H0
- IHC-P: Human endometrial carcinoma, and Human endometrial adenocarcinoma tissue; Flow Cyt: HL-60 cells.
This product is FOR RESEARCH USE ONLY. For commercial use, please contact firstname.lastname@example.org.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA
Concentration information loading...
PurityProtein A/G purified
Purification notesPurified from TCS by protein A/G.
Our Abpromise guarantee covers the use of ab227677 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Antigen retrieval: Boil tissue section in EDTA buffer, pH 8.0 for 10 minutes followed by cooling at room temperature for 20 minutes.
FunctionSubstrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Probably recognizes and binds to phosphorylated target proteins. Involved in the degradation of cyclin-E, MYC, NOTCH1 released notch intracellular domain (NICD), and probably PSEN1.
Tissue specificityIsoform 1 is widely expressed. Isoform 4 is expressed in brain.
Sequence similaritiesContains 1 F-box domain.
Contains 7 WD repeats.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationCytoplasm; Nucleus; Nucleus > nucleolus and Nucleus > nucleoplasm.
- Information by UniProt
- AGO antibody
- Archipelago antibody
- Archipelago homolog antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human endometrial adenocarcinoma tissue sections labeling Fbxw7 with ab227677 at 1/100 dilution (1.56 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Flow cytometric analysis of HL-60 (human promyelocytic leukemia cell line) cell line labeling Fbxw7 with ab227677 at 1/400 (green) compared with a rabbit isotype control (blue).
Formalin-fixed, paraffin-embedded human endometrial adenocarcinoma tissue stained for Fbxw7 with ab227677 at 1/100 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab227677 has not yet been referenced specifically in any publications.