Overview

  • Product name

    Anti-Fbxw7 antibody [SP237]
    See all Fbxw7 primary antibodies
  • Description

    Rabbit monoclonal [SP237] to Fbxw7
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human Fbxw7 aa 200-300 (internal sequence). The exact sequence is proprietary.
    Database link: Q969H0

  • Positive control

    • IHC-P: Human endometrial carcinoma, and Human endometrial adenocarcinoma tissue; FC: HL-60 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab227677 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/400.
IHC-P 1/100.

Antigen retrieval: Boil tissue section in EDTA buffer, pH 8.0 for 10 minutes followed by cooling at room temperature for 20 minutes.

Target

  • Function

    Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Probably recognizes and binds to phosphorylated target proteins. Involved in the degradation of cyclin-E, MYC, NOTCH1 released notch intracellular domain (NICD), and probably PSEN1.
  • Tissue specificity

    Isoform 1 is widely expressed. Isoform 4 is expressed in brain.
  • Sequence similarities

    Contains 1 F-box domain.
    Contains 7 WD repeats.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Cytoplasm; Nucleus; Nucleus > nucleolus and Nucleus > nucleoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • AGO antibody
    • Archipelago antibody
    • Archipelago homolog antibody
    • Archipelago, Drosophila, homolog of antibody
    • CDC4 antibody
    • CDC4, S.cerevisiae, homolog of antibody
    • DKFZp686F23254 antibody
    • F box and WD 40 domain protein 7 (archipelago homolog, Drosophila) antibody
    • F box and WD 40 domain protein 7 antibody
    • F box and WD repeat domain containing 7 antibody
    • F box protein FBW7 antibody
    • F box protein FBX30 antibody
    • F box protein SEL 10 antibody
    • F box protein SEL10 antibody
    • F-box and WD repeat domain containing 7, E3 ubiquitin protein ligase antibody
    • F-box and WD-40 domain-containing protein 7 antibody
    • F-box protein FBX30 antibody
    • F-box/WD repeat-containing protein 7 antibody
    • FBW6 antibody
    • FBW7 antibody
    • FBX30 antibody
    • FBXO30 antibody
    • FBXW6 antibody
    • FBXW7 antibody
    • FBXW7_HUMAN antibody
    • FLJ16457 antibody
    • hAgo antibody
    • hCdc4 antibody
    • Homolog of C elegans sel 10 antibody
    • Homolog of C.elegans sel10 antibody
    • SEL 10 antibody
    • SEL-10 antibody
    • SEL10 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human endometrial adenocarcinoma tissue sections labeling Fbxw7 with ab227677 at 1/100 dilution (1.56 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Flow cytometric analysis of HL-60 (human promyelocytic leukemia cell line) cell line labeling Fbxw7 with ab227677 at 1/400 (green) compared with a rabbit isotype control (blue).

  • Formalin-fixed, paraffin-embedded human endometrial adenocarcinoma tissue stained for Fbxw7 with ab227677 at 1/100 dilution in immunohistochemical analysis.

References

ab227677 has not yet been referenced specifically in any publications.

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