The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 5µl for 106 cells. ab91534-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
Binds to the Fc region of immunoglobulins epsilon. High affinity receptor. Responsible for initiating the allergic response. Binding of allergen to receptor-bound IgE leads to cell activation and the release of mediators (such as histamine) responsible for the manifestations of allergy. The same receptor also induces the secretion of important lymphokines.
Flow cytometric analysis of normal Human peripheral blood cells with Anti-Human IgE FITC and mouse IgG2b kappa Isotype Control APC (left) or ab155369 (right). Cells in the lymphocyte gate were used for analysis (note: basophils reside in FCS/SSC position of lymphocytes).