Biotin Anti-Fc epsilon RI/FCER1A antibody [CRA2] (ab122915)
Key features and details
- Biotin Mouse monoclonal [CRA2] to Fc epsilon RI/FCER1A
- Suitable for: Flow Cyt, WB, ICC/IF
- Reacts with: Human
- Conjugation: Biotin
- Isotype: IgG1
Overview
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Product name
Biotin Anti-Fc epsilon RI/FCER1A antibody [CRA2]
See all Fc epsilon RI/FCER1A primary antibodies -
Description
Biotin Mouse monoclonal [CRA2] to Fc epsilon RI/FCER1A -
Host species
Mouse -
Conjugation
Biotin -
Tested applications
Suitable for: Flow Cyt, WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human Fc epsilon RI/FCER1A aa 50-200.
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Positive control
- CHO/alpha beta gamma cells
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General notes
[biotin]/[IgG] = 6.9
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Ion Exchange Chromatography -
Purification notes
The IgG fraction was purified from serum free culture medium of mouse hybridoma by propriety chromatography under mild conditions. Filter sterilized. -
Clonality
Monoclonal -
Clone number
CRA2 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab122915 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt |
Use at an assay dependent concentration.
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WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 29 kDa.
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ICC/IF |
Use at an assay dependent concentration.
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Notes |
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Flow Cyt
Use at an assay dependent concentration. |
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 29 kDa. |
ICC/IF
Use at an assay dependent concentration. |
Target
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Function
Binds to the Fc region of immunoglobulins epsilon. High affinity receptor. Responsible for initiating the allergic response. Binding of allergen to receptor-bound IgE leads to cell activation and the release of mediators (such as histamine) responsible for the manifestations of allergy. The same receptor also induces the secretion of important lymphokines. -
Sequence similarities
Contains 2 Ig-like (immunoglobulin-like) domains. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 2205 Human
- Omim: 147140 Human
- SwissProt: P12319 Human
- Unigene: 897 Human
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Alternative names
- Fc epsilon RI alpha antibody
- Fc epsilon RI alpha chain antibody
- Fc epsilon RI alpha-chain antibody
see all
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab122915 has been referenced in 5 publications.
- Takai T et al. Direct expression of the extracellular portion of human FcepsilonRIalpha chain as inclusion bodies in Escherichia coli. Biosci Biotechnol Biochem 65:79-85 (2001). PubMed: 11272849
- Goto T et al. Enhanced expression of the high-affinity receptor for IgE (Fc(epsilon)RI) associated with decreased numbers of Langerhans cells in the lesional epidermis of atopic dermatitis. J Dermatol Sci 27:156-61 (2001). PubMed: 11641054
- Takai T et al. Epitope analysis and primary structures of variable regions of anti-human FcepsilonRI monoclonal antibodies, and expression of the chimeric antibodies fused with human constant regions. Biosci Biotechnol Biochem 64:1856-67 (2000). PubMed: 11055388
- Hasegawa S et al. Functional expression of the high affinity receptor for IgE (FcepsilonRI) in human platelets and its' intracellular expression in human megakaryocytes. Blood 93:2543-51 (1999). PubMed: 10194433
- Hakimi J et al. The alpha subunit of the human IgE receptor (FcERI) is sufficient for high affinity IgE binding. J Biol Chem 265:22079-81 (1990). PubMed: 2148316