Key features and details
- Rabbit polyclonal to Fc epsilon RI/FCER1A
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-Fc epsilon RI/FCER1A antibody
See all Fc epsilon RI/FCER1A primary antibodies
DescriptionRabbit polyclonal to Fc epsilon RI/FCER1A
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Mouse, Human
Recombinant fragment corresponding to Human Fc epsilon RI/FCER1A aa 26-205.
VPQKPKVSLNPPWNRIFKGENVTLTCNGNNFFEVSSTKWFHNGSLSEETN SSLNIVNAKFEDSGEYKCQHQQVNESEPVYLEVFSDWLLLQASAEVVMEG QPLFLRCHGWRNWDVYKVIYYKDGEALKYWYENHNISITNATVEDSGTYY CTGKVWQLDYESEPLNITVIKAPREKYWLQ
Database link: P12319
- IHC-P: Human prostate cancer and skin tissues. ICC/IF: HeLa cells. WB: Mouse heart and brain lysates.
This product was previously labelled as Fc epsilon RI
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab229889 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/50 - 1/200.|
|IHC-P||1/20 - 1/200.|
|WB||1/2000 - 1/5000. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa).|
FunctionBinds to the Fc region of immunoglobulins epsilon. High affinity receptor. Responsible for initiating the allergic response. Binding of allergen to receptor-bound IgE leads to cell activation and the release of mediators (such as histamine) responsible for the manifestations of allergy. The same receptor also induces the secretion of important lymphokines.
Sequence similaritiesContains 2 Ig-like (immunoglobulin-like) domains.
Cellular localizationCell membrane.
- Information by UniProt
- Fc epsilon RI alpha antibody
- Fc epsilon RI alpha chain antibody
- Fc epsilon RI alpha-chain antibody
All lanes : Anti-Fc epsilon RI/FCER1A antibody (ab229889) at 1/2000 dilution
Lane 1 : Mouse heart lysate
Lane 2 : Mouse brain lysate
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 30 kDa
Observed band size: 30 kDa
HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Fc epsilon RI/FCER1A (green) using ab229889 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).
Paraffin-embedded human prostate cancer tissue stained for Fc epsilon RI/FCER1A using ab229889 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human skin tissue stained for Fc epsilon RI/FCER1A using ab229889 at 1/100 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab229889 has not yet been referenced specifically in any publications.